前列腺素D2对NG108 - 15杂交细胞与肌肉细胞间突触传递的促进作用。

Facilitation of synaptic transmission by prostaglandin D2 at synapses between NG108-15 hybrid and muscle cells.

作者信息

Higashida H, Nakagawa Y, Miki N

出版信息

Brain Res. 1984 Mar 12;295(1):113-9. doi: 10.1016/0006-8993(84)90821-7.

Abstract

The effect of prostaglandin (PG) D2 on neuronal functions was investigated in neuroblastoma X glioma NG108-15 hybrid cells. PGD2 caused a sustained increase in miniature end-plate potentials (MEPPs) recorded from cultured striated muscle cells which had formed junctions with NG108-15 cells. PGD2 initially hyperpolarized and then depolarized NG108-15 cells. The time course of depolarization fitted well to the facilitative phase of MEPPs. The same action on synaptic transmission and membrane potentials was detected with PGF2 alpha but not with PGE1. PGD2 (10(-4)M) produced a 3-fold increase of adenylate cyclase activity in NG108-15 cell homogenates through its receptors that are distinct from those of PGE1 and PGI2. These results show that PGD2 facilitates MEPP frequency from NG108-15 cells due to depolarization, and suggest that PGD2 may act as a physiological neuromodulator for synaptic transmission in vivo.

摘要

在神经母细胞瘤X胶质瘤NG108 - 15杂交细胞中研究了前列腺素（PG）D2对神经元功能的影响。PGD2使与NG108 - 15细胞形成连接的培养横纹肌细胞记录的微小终板电位（MEPPs）持续增加。PGD2最初使NG108 - 15细胞超极化，然后使其去极化。去极化的时间进程与MEPPs的易化相非常吻合。用PGF2α检测到对突触传递和膜电位有相同作用，但PGE1未检测到。PGD2（10⁻⁴M）通过其与PGE1和PGI2不同的受体使NG108 - 15细胞匀浆中的腺苷酸环化酶活性增加3倍。这些结果表明，PGD2由于去极化而促进NG108 - 15细胞的MEPP频率，并提示PGD2可能在体内作为突触传递的生理性神经调节剂起作用。