多环芳烃酚类与DNA的微粒体介导反应。

Microsome-mediated reactions of phenols of polycyclic hydrocarbons with DNA.

作者信息

Hewer A, Phillips D H, Hodgson R M, Grover P L

出版信息

Cancer Lett. 1984 Apr;22(3):321-8. doi: 10.1016/0304-3835(84)90169-1.

Abstract

Phenolic metabolites of 7 polycyclic hydrocarbons were incubated with rat-liver microsomal fractions in the presence of DNA. Hydrocarbon-nucleoside adducts with chromatographic properties similar to those of diol-epoxide-deoxyribonucleoside adducts, were detected in hydrolysates of DNA that had been incubated with phenols of benzo[a]pyrene, benz[a]anthracene and chrysene. Adducts were not detected when phenols of phenanthrene, pyrene, dibenz[a,c]anthracene or dibenz[a,h]anthracene were further metabolised. The possible contribution of phenolic metabolites to the carcinogenic activity and DNA binding of polycyclic hydrocarbons is discussed.

摘要

在存在DNA的情况下，将7种多环烃的酚类代谢物与大鼠肝脏微粒体组分一起孵育。在与苯并[a]芘、苯并[a]蒽和 Chrysene 的酚类一起孵育的DNA水解物中，检测到具有与二醇-环氧化物-脱氧核糖核苷加合物相似色谱性质的烃-核苷加合物。当菲、芘、二苯并[a,c]蒽或二苯并[a,h]蒽的酚类进一步代谢时，未检测到加合物。讨论了酚类代谢物对多环烃致癌活性和DNA结合的可能贡献。