Shumilov V Iu
Mol Biol (Mosk). 1984 Mar-Apr;18(2):525-40.
A circular periodic map of short palindromic DNA sequences is constructed using the sequence homology and symmetry. It is applied to compare sequences recognised by class II restriction and modification enzymes with other similar DNA sequences. All known restriction sites have two strong properties: they are enriched by GC pairs, and clustered (purine-purine, pyrimidine-pyrimidine) bonds predominant upon alternating ones. The preference of AT/GC alternation is only slight. These properties were compared quantitatively with the help of suggested numerical methods among different groups of restriction enzymes. The map is applied for prediction of new specificities of restriction modification systems. Possible mechanism of DNA sequence recognition by these enzymes and their evolution are discussed.
利用序列同源性和对称性构建了短回文DNA序列的环状周期图。它被用于比较II类限制酶和修饰酶识别的序列与其他相似DNA序列。所有已知的限制位点都有两个显著特性：它们富含GC碱基对，并且聚类（嘌呤-嘌呤、嘧啶-嘧啶）键比交替键占主导。AT/GC交替的偏好性很小。借助建议的数值方法，在不同组的限制酶之间对这些特性进行了定量比较。该图用于预测限制修饰系统的新特异性。讨论了这些酶识别DNA序列的可能机制及其进化。
