Conner M, Kalica A, Kita J, Quick S, Schiff E, Joubert J, Gillespie J
Vet Microbiol. 1984 Feb;9(1):15-25. doi: 10.1016/0378-1135(84)90075-0.
Reoviruses have been isolated from a number of species including human, bovine, feline, canine and equine. In most species they seem to produce mild to inapparent disease. We have isolated a reovirus type 3 from a foal with diarrhea. The virus designated the Ralph strain has been propagated in both the MA-104 and A-72 cell lines. The strain produced cytoplasmic inclusion bodies in these cell cultures. Tissue-cultured virus fixed complement in the presence of reovirus antibodies, but failed to do so in the presence of rotavirus antiserum. By electron microscopy the viral particle measured +/- 65 nm. The virus hemagglutinated pig erythrocytes, but not human O, human A, calf, cow, chicken or guinea pig erythrocytes. In the hemagglutination test there was complete reciprocal crossing between the Ralph strain and the NIH reovirus type 3, but there was no crossing with the NIH reovirus types 1 and 2. A limited serological survey was completed on serum samples from New York State horses collected in 1976-1977 and 1981 using the hemagglutination-inhibition test. The percentage with antibodies to reovirus types 1, 2 and 3 for 1976-1977 was 24.5, 42.2 and 3.9% and in 1981, 8.8, 9.8 and 3.9%, respectively.
呼肠孤病毒已从包括人类、牛、猫、犬和马在内的多种物种中分离出来。在大多数物种中，它们似乎引起轻度至隐性疾病。我们从一匹患腹泻的马驹中分离出一株3型呼肠孤病毒。命名为拉尔夫毒株的病毒已在MA - 104和A - 72细胞系中传代培养。该毒株在这些细胞培养物中产生细胞质包涵体。组织培养病毒在呼肠孤病毒抗体存在时能固定补体，但在轮状病毒抗血清存在时则不能。通过电子显微镜观察，病毒粒子大小约为±65纳米。该病毒能凝集猪红细胞，但不能凝集人O型、人A型、小牛、奶牛、鸡或豚鼠的红细胞。在血凝试验中，拉尔夫毒株与国立卫生研究院3型呼肠孤病毒完全相互交叉，但与国立卫生研究院1型和2型呼肠孤病毒无交叉反应。使用血凝抑制试验对1976 - 1977年和1981年采集的纽约州马匹血清样本进行了有限的血清学调查。1976 - 1977年针对1型、2型和3型呼肠孤病毒有抗体的百分比分别为24.5%、42.2%和3.9%，1981年分别为8.8%、9.8%和3.9%。
