Erasmus R T, Osotimehin B, Ugbode C, Famuyiwa O O
Afr J Med Med Sci. 1983 Sep-Dec;12(3-4):177-82.
Glycosylation of haemoglobin was evaluated in 30 normal adult Nigerians and 60 diabetic adult Nigerians who were at various stages of blood glucose control, by measuring ketoamine-linked hexoses in haemoglobin. These hexoses were converted by digestion with 1 M oxalic acid for 4.5 h at 100 degrees C to 5-hydroxymethylfurfural, which was measured by reaction with 2 thiobarbituric acid. Glycosylation of haemoglobin was expressed as micromoles (mumol) of fructose per 5% haemoglobin. A mean fructose concentration of 91.0 mumol/1 (s.d. +/- 9.3) was obtained for normal subjects; that for diabetic adult Nigerians was 175.3 mumol/l (s.d. +/- 26.4). There was significant correlation between fasting blood glucose levels and glycosylated haemoglobins in both normal adults (r = 0.77, P less than 0.001) and diabetic adults (r = 0.90, P less than 0.001). The colorimetric method was found to be precise and accurate with an interassay variability of 1.44% and an intra-assay variability of 0.98%.
通过测量血红蛋白中与酮胺相连的己糖，对30名正常成年尼日利亚人和60名处于血糖控制不同阶段的成年糖尿病尼日利亚人进行血红蛋白糖基化评估。这些己糖在100℃下用1M草酸消化4.5小时后转化为5-羟甲基糠醛，5-羟甲基糠醛通过与2-硫代巴比妥酸反应进行测量。血红蛋白糖基化以每5%血红蛋白中果糖的微摩尔数（μmol）表示。正常受试者的果糖平均浓度为91.0μmol/l（标准差±9.3）；成年糖尿病尼日利亚人的果糖平均浓度为175.3μmol/l（标准差±26.4）。正常成年人（r = 0.77，P < 0.001）和糖尿病成年人（r = 0.90，P < 0.001）的空腹血糖水平与糖化血红蛋白之间均存在显著相关性。发现比色法精确且准确，批间变异率为1.44%，批内变异率为0.98%。
