Nazerian K, Witter R L
Avian Dis. 1984 Jan-Mar;28(1):160-7.
Continuous passage of MDCC-RP1, a highly tumorigenic Marek's disease (MD) lymphoblastoid cell line, in cell culture resulted in a gradual loss in ability of the cell line to cause progressive tumors in susceptible day-old chicks. Inoculation of day-old chicks with high-cell-culture-passaged (187th to 417th) nontumorigenic MDCC-RP1 cells gave excellent protection against challenge at 8 days with low-passaged tumorigenic MDCC-RP1 cells but failed to protect against primary tumors caused by inoculation with MD virus. Vaccination with the herpesvirus of turkeys, on the other hand, protected the chickens well against primary tumors caused by MD virus and against transplantable tumors caused by tumorigenic MDCC-RP1 cells, but it did not protect as well against another MD lymphoblastoid cell line, MDCC-RP4. It is unlikely, therefore, that vaccines prepared from passaged MDCC-RP1 cell lines will have value for protecting chickens against MD in the field.
高度致瘤性的马立克氏病（MD）淋巴母细胞系MDCC-RP1在细胞培养中连续传代，导致该细胞系在易感一日龄雏鸡中引发进行性肿瘤的能力逐渐丧失。用高细胞培养传代（第187代至417代）的非致瘤性MDCC-RP1细胞接种一日龄雏鸡，能有效保护其在8日龄时免受低传代致瘤性MDCC-RP1细胞的攻击，但无法预防接种MD病毒引起的原发性肿瘤。另一方面，用火鸡疱疹病毒疫苗接种，能很好地保护鸡免受MD病毒引起的原发性肿瘤以及致瘤性MDCC-RP1细胞引起的可移植肿瘤的侵害，但对另一种MD淋巴母细胞系MDCC-RP4的保护效果不佳。因此，由传代MDCC-RP1细胞系制备的疫苗在实际中保护鸡免受MD侵害的价值不大。
