Dave J R, Witorsch R J
Biochim Biophys Acta. 1984 May 30;772(3):321-7. doi: 10.1016/0005-2736(84)90149-4.
The objectives of this study were to determine (i) if the age-related changes in 125I-labeled ovine prolactin specific binding of rat ventral prostate was correlated with changes in membrane lipid microviscosity and (ii) if membrane fluidizers produced age-dependent effects on prolactin binding of prostatic membranes. The degree of fluidization was monitored by a fluorescence polarization method using 1,6-diphenylhexatriene. Membrane preparations of ventral prostate glands obtained from immature (24-25 days old), young-adult (80-90 days old) and aged (550-610 days old) male rats were used for prolactin binding and membrane lipid microviscosity measurements. Relative to immature rats, prostatic prolactin binding decreased approximately 50% in young-adult rats and 75% in aged rats. Membrane lipid microviscosity, relative to immature rats, was increased 72% in young-adult rats and 140% in aged rats. Prostatic membranes obtained from immature animals exhibited no significant effects of in vitro alcohol treatment on prolactin binding, whereas, those obtained from aged animals exhibited maximal increase in prolactin binding. The value of the microviscosity parameter, after in vitro alcohol exposure, exhibited no significant changes in immature animals, whereas, this parameter was decreased approximately 15% in young-adults and approximately 30% in aged animals. These data suggest that in vitro fluidization of prostatic membrane exhibits an age-dependent modification of prolactin binding.
（i）大鼠腹侧前列腺中¹²⁵I标记的绵羊催乳素特异性结合的年龄相关变化是否与膜脂质微粘度的变化相关；（ii）膜流化剂是否对前列腺膜的催乳素结合产生年龄依赖性影响。使用1,6 - 二苯基己三烯通过荧光偏振法监测流化程度。从幼年（24 - 25日龄）、青年成年（80 - 90日龄）和老年（550 - 610日龄）雄性大鼠获得的腹侧前列腺膜制剂用于催乳素结合和膜脂质微粘度测量。相对于幼年大鼠，青年成年大鼠前列腺催乳素结合减少约50%，老年大鼠减少75%。相对于幼年大鼠，青年成年大鼠膜脂质微粘度增加72%，老年大鼠增加140%。从未成熟动物获得的前列腺膜在体外酒精处理对催乳素结合无显著影响，而从老年动物获得的前列腺膜催乳素结合增加最大。体外酒精暴露后，微粘度参数值在未成熟动物中无显著变化，而在青年成年动物中该参数降低约15%，在老年动物中降低约30%。这些数据表明前列腺膜的体外流化对催乳素结合表现出年龄依赖性修饰。
