Stel'makh L N, Rozhmanova O M, Lishko V K
Biokhimiia. 1984 Mar;49(3):460-3.
A procedure for incorporation of isolated cattle brain Na,K-ATPase into erythrocyte membranes by proteoliposomes has been elaborated. The Na,K-ATPase activity of proteoliposome-treated human erythrocytes containing incorporated Na,K-ATPase does not exceed that of control erythrocytes. In the erythrocyte membrane the incorporated enzyme exists in a functionally active state and retains the vector properties of the Na+-pump. Exogenous ATP stimulates 22Na influx and 86Rb efflux in and from the erythrocytes.
已制定了一种通过蛋白脂质体将分离的牛脑钠钾ATP酶整合到红细胞膜中的方法。含有整合钠钾ATP酶的经蛋白脂质体处理的人红细胞的钠钾ATP酶活性不超过对照红细胞。在红细胞膜中，整合的酶以功能活性状态存在，并保留钠泵的载体特性。外源性ATP刺激红细胞内外的22Na流入和86Rb流出。
