Mishin V M, Makarova S I, Gutkina N I, Grishanova A Iu, Liakhovich V V
Biokhimiia. 1984 Mar;49(3):464-9.
Using antibodies against electrophoretically homogeneous cytochrome P-448 from rat liver microsomes induced by 3-methylcholanthrene, the changes in the immunologic identity and contents by cytochrome P-448 induced by 3-methylcholanthrene, 3.4-benzpyrene and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), were studied. No cytochrome P-448 was detected in the liver microsomes of control or phenobarbital-induced rats. This form of the cytochrome makes up to about 35% of the total content of the CO-binding hemoprotein during TCDD induction and up to 90% during 3-methylcholanthrene and 3,4-benzpyrene induction. On the other hand, 3-methylcholanthrene, 3,4-benzpyrene and TCDD significantly and equally activates the cytochrome P-448-dependent benzpyrene hydroxylase, since the antibodies against cytochrome P-448 inhibit benzpyrene metabolism in the microsomes by 85-90%. The possible reasons for the TCDD-induced increase in the catalytic activity of cytochrome P-448 as compared to the immunologically identical cytochrome P-448 induced by 3-methylcholanthrene and 3,4-benzpyrene, are discussed.
利用针对由3-甲基胆蒽诱导的大鼠肝脏微粒体中电泳纯的细胞色素P-448的抗体，研究了由3-甲基胆蒽、3,4-苯并芘和2,3,7,8-四氯二苯并对二恶英（TCDD）诱导的细胞色素P-448的免疫学特性和含量变化。在对照或苯巴比妥诱导的大鼠肝脏微粒体中未检测到细胞色素P-448。在TCDD诱导过程中，这种细胞色素形式占CO结合血红蛋白总含量的约35%，在3-甲基胆蒽和3,4-苯并芘诱导过程中占90%。另一方面，3-甲基胆蒽、3,4-苯并芘和TCDD均能显著且同等程度地激活细胞色素P-448依赖性苯并芘羟化酶，因为针对细胞色素P-448的抗体可使微粒体中的苯并芘代谢抑制85%-90%。讨论了与由3-甲基胆蒽和3,4-苯并芘诱导的免疫学特性相同的细胞色素P-448相比，TCDD诱导的细胞色素P-448催化活性增加的可能原因。
