Haque S J, Poddar M K
Biosci Rep. 1984 Mar;4(3):239-43. doi: 10.1007/BF01119659.
There is no shift of emission maximum (F470nm) of bovine serum albumin (BSA)-1-anilino-8-naphthalene sulphonic acid (ANS) complex in the presence of delta-9-tetrahydrocannabinol (delta-9-THC) alone and cannabidiol (CBD) or cannabinol (CBN) in the presence and absence of delta-9-THC. Delta-9-THC (1.66-13.33 microM) and CBD at higher concentrations (13.33-20.0 microM) produce a concentration-dependent significant quenching of fluorescence of BSA-ANS complex, but CBN (1.66-20.0 microM) as well as CBD at lower concentrations (1.66-6.66 microM) fails to produce any significant change in fluorescence intensity under similar conditions. Furthermore, neither CBD nor CBN is able to affect the delta-9-THC-induced quenching of fluorescence intensity of BSA-ANS complex. These results indicate that the binding of cannabinoids to the ANS binding sites of BSA molecule are in the order delta-9-THC greater than CBD greater than CBN, and CBD or CBN does not have any influence on the binding of delta-9-THC to BSA molecules under these conditions.
单独存在δ-9-四氢大麻酚（δ-9-THC）时，以及在有和没有δ-9-THC的情况下，牛血清白蛋白（BSA）-1-苯胺基-8-萘磺酸（ANS）复合物的发射最大值（F470nm）均无变化。δ-9-THC（1.66 - 13.33微摩尔）和较高浓度（13.33 - 20.0微摩尔）的大麻二酚（CBD）会使BSA-ANS复合物的荧光产生浓度依赖性的显著猝灭，但大麻酚（CBN）（1.66 - 20.0微摩尔）以及较低浓度（1.66 - 6.66微摩尔）的CBD在类似条件下不会使荧光强度产生任何显著变化。此外，CBD和CBN均不能影响δ-9-THC诱导的BSA-ANS复合物荧光强度的猝灭。这些结果表明，在这些条件下，大麻素与BSA分子的ANS结合位点的结合顺序为δ-9-THC大于CBD大于CBN，并且CBD或CBN对δ-9-THC与BSA分子的结合没有任何影响。
