Kling D, Becker M M, Kruth H S, Gamble W
Environ Res. 1984 Jun;34(1):87-102. doi: 10.1016/0013-9351(84)90078-1.
2,4,5,2',4',5'-Hexachlorobiphenyl (HCB) induced cytoplasmic inclusions and lipidosis in normal ( AG1437 ) and hypercholesterolemic ( GM488 ) human skin fibroblasts. Quantitative and qualitative microsocopic fluorescence analysis showed that the cytoplasmic inclusions are formed as early as 3 hr after treatment with HCB. The inclusions contain lipids but no detectable nonesterified cholesterol or cholesteryl ester. High density lipoproteins (HDL), low density lipoproteins (LDL), and very low density lipoproteins (VLDL) facilitate the apparent uptake of HCB by skin fibroblasts. HDL and LDL appeared to reverse the induction of cytoplasmic inclusions and lipidosis when cells were pretreated with HCB, the HCB was removed from media, and the cells were incubated with LDL or HDL. The results suggest that lipoproteins participate in the uptake and egress of HCB from skin fibroblasts.
2,4,5,2',4',5'-六氯联苯（HCB）在正常（AG1437）和高胆固醇血症（GM488）的人皮肤成纤维细胞中诱导细胞质包涵体和脂肪变性。定量和定性显微镜荧光分析表明，在用HCB处理后3小时就开始形成细胞质包涵体。这些包涵体含有脂质，但未检测到游离胆固醇或胆固醇酯。高密度脂蛋白（HDL）、低密度脂蛋白（LDL）和极低密度脂蛋白（VLDL）促进皮肤成纤维细胞对HCB的明显摄取。当细胞先用HCB预处理，然后从培养基中去除HCB，再与LDL或HDL一起孵育时，HDL和LDL似乎能逆转细胞质包涵体和脂肪变性的诱导。结果表明，脂蛋白参与了HCB在皮肤成纤维细胞中的摄取和排出。
