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Particulate cyclic 3',5'-nucleotide phosphodiesterase and calmodulin of cardiac muscle.

作者信息

Ahluwalia G, Rhoads A R, Lulla M

出版信息

Int J Biochem. 1984;16(5):483-8. doi: 10.1016/0020-711x(84)90164-2.

DOI:10.1016/0020-711x(84)90164-2
PMID:6327416
Abstract

Cyclic AMP and cyclic GMP phosphodiesterase and calmodulin were measured in purified subcellular fractions of cardiac muscle. Phosphodiesterase activity solubilized by sonication of the nuclear fraction yielded a major 6.6 S form which was calcium-sensitive and cyclic GMP-specific. Phosphodiesterase activity occurring in the nuclear fraction could be further enriched by subfractionation on sucrose density gradients in the presence of MgCl2.

摘要

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