使用克隆的DNA片段分析肺炎链球菌转化中供体DNA的命运。
Use of a cloned DNA fragment to analyze the fate of donor DNA in transformation of Streptococcus pneumoniae.
作者信息
Méjean V, Claverys J P
出版信息
J Bacteriol. 1984 Jun;158(3):1175-8. doi: 10.1128/jb.158.3.1175-1178.1984.
Abstract
The integration of donor label into the recipient fragment is followed during transformation of Streptococcus pneumoniae. The method used involves gel analysis of restriction endonuclease-treated recipient DNA after recombination with a radioactively labeled homologous cloned fragment.
摘要
在肺炎链球菌转化过程中，对供体标签整合到受体片段的情况进行了跟踪。所采用的方法包括在与放射性标记的同源克隆片段重组后，对经限制性内切酶处理的受体DNA进行凝胶分析。
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