肺炎球菌转化中供体脱氧核糖核酸标记活性从隐蔽期的恢复
Recovery of donor deoxyribonucleic acid marker activity from eclipse in pneumococcal transformation.
作者信息
Ghei O K, Lacks S A
出版信息
J Bacteriol. 1967 Mar;93(3):816-29. doi: 10.1128/jb.93.3.816-829.1967.
Abstract
After the uptake of deoxyribonucleic acid (DNA), donor marker-transforming activity is temporarily lost. Restoration of the activity by annealing in vitro supports the idea that donor DNA is single-stranded at this stage. Kinetics of in vivo recovery from eclipse were examined for various markers at three temperatures. Sigmoidal recovery curves at lower temperatures indicate that the process consists of several steps. Rate of recovery was found to depend on the nature of the donor marker. Single-site markers recover much more rapidly than multisite markers corresponding to recipient deletions. Single-site markers vary somewhat in recovery rate, with rapidity of recovery inversely related to integration efficiency. Appearance of a recombinant-transforming activity lags only slightly behind recovery of its constituent donor marker.
摘要
在摄取脱氧核糖核酸（DNA）后，供体标记物的转化活性会暂时丧失。通过体外退火恢复活性支持了供体DNA在此阶段为单链的观点。在三个温度下，针对各种标记物研究了体内从隐蔽期恢复的动力学。较低温度下的S形恢复曲线表明该过程由几个步骤组成。发现恢复速率取决于供体标记物的性质。单位点标记物的恢复速度比与受体缺失相对应的多位点标记物快得多。单位点标记物的恢复速率有所不同，恢复速度与整合效率呈负相关。重组转化活性的出现仅比其组成供体标记物的恢复略微滞后。
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