Tn5.7 construction and physical mapping of pRPS404 containing photosynthetic genes from Rhodopseudomonas capsulata.

A transposon, Tn5.7, has been constructed incorporating the transposition functions of Tn5 and the antibiotic-resistance factors from Tn7. It was used to mutagenize the plasmid pRPS404 which contains the photosynthetic genes of Rhodopseudomonas capsulata and is KanamycinR . In conjunction with the mutagenesis, physical mapping of the restriction endonuclease recognition sites for XhoI, BglII, KpnI, and SstI has been accomplished.