[Inhibiting effect of colcemid on the development of cytopathic changes due to the Sindbis virus].

The model of Chinese hamster (Cricetulus griseus) cell line infected with Sindbus virus was used to show that the presence in the maintenance medium of 0.01-0.1 micrograms/ml of colcemide inhibited the development of cytopathic changes, accumulation of viral antigens, and virus-specific complement-dependent cytolysis without affecting infectious virus production. The addition to the medium of 0.5% immune serum to Sindbis virus resulted not only in a similar inhibition but in a decrease of infectious virus titres. The latter effect was observed upon the addition of both colcemide and antiserum. Possible mechanisms of inhibition are discussed.