• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

ATP和环磷酸腺苷对大鼠下丘脑组氨酸脱羧酶活性的体外调节：磷酸化条件下的酶失活

Regulation of histidine decarboxylase activity in rat hypothalamus in vitro by ATP and cyclic AMP: enzyme inactivation under phosphorylating conditions.

作者信息

Huszti Z, Magyar K

出版信息

Agents Actions. 1984 Apr;14(3-4):546-9. doi: 10.1007/BF01973868.

DOI:10.1007/BF01973868
PMID:6328955
Abstract

In vitro, hypothalamic HD1 from rat, could strikingly be inhibited by ATP and cyclic AMP. The enzyme inhibition is partially dependent upon Mg2+ and the circumstances favourable for a cAMP-dependent phosphorylation. An almost complete inhibition could be achieved by incubating the homogenate of the hypothalamus under phosphorylating conditions (ATP, cAMP, Mg2+ and IBMX) in the presence of a cAMP-dependent protein kinase (obtained from bovine thymus). Cyclic nucleotides and ATP alone elicit only moderate inhibitions on the hypothalamic HD activity. Neither ATP, nor cAMP, added alone or in combinations, alter the total brain or the hypothalamic HNMT from guinea-pigs or rats in concentrations up to 10(-3) M. Results suggest that hypothalamic HD is regulated through a cAMP-dependent process, probable a direct phosphorylation, via a cAMP-dependent protein kinase.

摘要

在体外，大鼠下丘脑的HD1可被ATP和环磷酸腺苷显著抑制。该酶的抑制作用部分依赖于Mg2+以及有利于环磷酸腺苷依赖性磷酸化的条件。通过在存在环磷酸腺苷依赖性蛋白激酶（从牛胸腺获得）的情况下，在磷酸化条件（ATP、环磷酸腺苷、Mg2+和异丁基甲基黄嘌呤）下孵育下丘脑匀浆，几乎可以实现完全抑制。单独的环核苷酸和ATP对下丘脑HD活性仅产生中度抑制。单独或组合添加的ATP和环磷酸腺苷，在浓度高达10(-3) M时，均不会改变豚鼠或大鼠的全脑或下丘脑HNMT。结果表明，下丘脑HD是通过环磷酸腺苷依赖性过程调节的，可能是通过环磷酸腺苷依赖性蛋白激酶进行直接磷酸化。

相似文献

1
Regulation of histidine decarboxylase activity in rat hypothalamus in vitro by ATP and cyclic AMP: enzyme inactivation under phosphorylating conditions.ATP和环磷酸腺苷对大鼠下丘脑组氨酸脱羧酶活性的体外调节：磷酸化条件下的酶失活
Agents Actions. 1984 Apr;14(3-4):546-9. doi: 10.1007/BF01973868.
2
Evidence for the role of cAMP-dependent protein kinase in the down-regulation of hypothalamic HD: reversal of cAMP-(ATP) induced inhibition of HD activity by the 'Walsh' inhibitor of cAMP-dependent protein kinase and by cyclic GMP.环磷酸腺苷（cAMP）依赖性蛋白激酶在下丘脑激素脱碘酶（HD）下调中的作用证据：环磷酸腺苷依赖性蛋白激酶的“沃尔什”抑制剂以及环磷酸鸟苷（cGMP）可逆转cAMP-（ATP）诱导的HD活性抑制。
Agents Actions. 1985 Apr;16(3-4):240-3. doi: 10.1007/BF01983150.
3
Stimulation of hypothalamic histidine decarboxylase by calcium-calmodulin and protein kinase (cAMP-dependent) inhibitor.
Agents Actions. 1987 Apr;20(3-4):233-5. doi: 10.1007/BF02074678.
4
Possible regulation of hypothalamus and lung histidine decarboxylase activity by cAMP-dependent protein kinase.环磷酸腺苷依赖性蛋白激酶对下丘脑和肺组织中组氨酸脱羧酶活性的潜在调节作用。
Eur J Biochem. 1991 Apr 10;197(1):191-6. doi: 10.1111/j.1432-1033.1991.tb15898.x.
5
In vitro inhibition of rat hypothalamus histidine decarboxylase by a specific inhibitor--4-imidazolyl-3-amino-2-butanone (McN-A-1293).
Biochem Pharmacol. 1978;27(24):2977-8. doi: 10.1016/0006-2952(78)90224-1.
6
Relationship between the multiple forms of rat gastric histidine decarboxylase: effects of conditions favouring phosphorylation and dephosphorylation.大鼠胃组织中组氨酸脱羧酶多种形式之间的关系：有利于磷酸化和去磷酸化条件的影响
Biochem Int. 1990;20(2):363-74.
7
Inhibition of histidine decarboxylase. Derivatives of histidine.
J Med Chem. 1977 Apr;20(4):506-9. doi: 10.1021/jm00214a009.
8
Activation of rat liver cholesterol ester hydrolase by cAMP-dependent protein kinase and protein kinase C.环磷酸腺苷（cAMP）依赖性蛋白激酶和蛋白激酶C对大鼠肝脏胆固醇酯水解酶的激活作用。
Lipids. 1989 Aug;24(8):733-6. doi: 10.1007/BF02535213.
9
Inhibition of histamine synthesis in brain by alpha-fluoromethylhistidine, a new irreversible inhibitor: in vitro and in vivo studies.新型不可逆抑制剂α-氟甲基组氨酸对脑内组胺合成的抑制作用：体内外研究
J Neurochem. 1980 Nov;35(5):1045-52. doi: 10.1111/j.1471-4159.1980.tb07858.x.
10
Cyclic AMP-dependent protein phosphorylation and insulin secretion in intact islets of Langerhans.朗格汉斯胰岛完整组织中环磷酸腺苷依赖性蛋白磷酸化与胰岛素分泌
Biochem J. 1984 Feb 15;218(1):87-99. doi: 10.1042/bj2180087.

引用本文的文献

1
Effects of various compounds on histidine decarboxylase activity: active site mapping.各种化合物对组氨酸脱羧酶活性的影响：活性位点图谱分析
Agents Actions. 1985 Oct;17(1):32-7. doi: 10.1007/BF01966677.
2
Stimulation of hypothalamic histidine decarboxylase by calcium-calmodulin and protein kinase (cAMP-dependent) inhibitor.
Agents Actions. 1987 Apr;20(3-4):233-5. doi: 10.1007/BF02074678.
3
Histamine, histidine decarboxylase and histamine-N-methyltransferase in brain areas of spontaneously hypertensive rats.自发性高血压大鼠脑区中的组胺、组氨酸脱羧酶和组胺-N-甲基转移酶

本文引用的文献

1
Regulation of histamine synthesis: altered synthesis and level of histamine in the hypothalamus of rats by repeated administration of histamine H1 and H2 receptor antagonists.
Agents Actions. 1980 Apr;10(1 Pt 2):98-100. doi: 10.1007/BF02024186.
2
Regulatory influence of histamine receptor activation and inhibition on the synthesis and level of hypothalamic histamine.
Agents Actions. 1984 Jun;14(5-6):580-9. doi: 10.1007/BF01978890.
3
Isotopic microassay of histamine, histidine, histidine decarboxylase and histamine methyltransferase in brain tissue.脑组织中组胺、组氨酸、组氨酸脱羧酶和组胺甲基转移酶的同位素微量测定法。
J Neurochem. 1972 May;19(5):1343-58. doi: 10.1111/j.1471-4159.1972.tb01459.x.
Naunyn Schmiedebergs Arch Pharmacol. 1988 Nov;338(5):573-6. doi: 10.1007/BF00179332.
4
Evidence for the role of cAMP-dependent protein kinase in the down-regulation of hypothalamic HD: reversal of cAMP-(ATP) induced inhibition of HD activity by the 'Walsh' inhibitor of cAMP-dependent protein kinase and by cyclic GMP.环磷酸腺苷（cAMP）依赖性蛋白激酶在下丘脑激素脱碘酶（HD）下调中的作用证据：环磷酸腺苷依赖性蛋白激酶的“沃尔什”抑制剂以及环磷酸鸟苷（cGMP）可逆转cAMP-（ATP）诱导的HD活性抑制。
Agents Actions. 1985 Apr;16(3-4):240-3. doi: 10.1007/BF01983150.
5
Characterization and expression of the complementary DNA encoding rat histidine decarboxylase.编码大鼠组氨酸脱羧酶的互补DNA的表征与表达
Proc Natl Acad Sci U S A. 1990 Jan;87(2):733-7. doi: 10.1073/pnas.87.2.733.
4
Cyclic AMP.环磷酸腺苷
Annu Rev Biochem. 1968;37:149-74. doi: 10.1146/annurev.bi.37.070168.001053.
5
Regulation of catecholamine synthesis in the rat brain in vitro by cyclic AMP.环磷酸腺苷对大鼠脑内儿茶酚胺合成的体外调节作用
Nature. 1974 Nov 8;252(5479):156-8. doi: 10.1038/252156a0.
6
Histamine-sensitive adenylate cyclase in hypothalamus of rat brain: H1 and H2 receptors.大鼠下丘脑组胺敏感腺苷酸环化酶：H1和H2受体
J Neurochem. 1978 Dec;31(6):1371-4. doi: 10.1111/j.1471-4159.1978.tb06562.x.
7
Cyclic AMP-dependent protein kinase mediates a cyclic AMP-stimulated decrease in ornithine and S-adenosylmethionine decarboxylase activities.环磷酸腺苷依赖性蛋白激酶介导环磷酸腺苷刺激的鸟氨酸和S-腺苷甲硫氨酸脱羧酶活性降低。
Proc Natl Acad Sci U S A. 1978 Feb;75(2):862-5. doi: 10.1073/pnas.75.2.862.
8
Direct phosphorylation of brain tyrosine hydroxylase by cyclic AMP-dependent protein kinase: mechanism of enzyme activation.环磷酸腺苷依赖性蛋白激酶对脑酪氨酸羟化酶的直接磷酸化作用：酶激活机制
Proc Natl Acad Sci U S A. 1978 Oct;75(10):4744-8. doi: 10.1073/pnas.75.10.4744.
9
Stimulation of tyrosine hydroxylase activity by cyclic AMP in synaptosomes and in soluble striatal enzyme preparations.环磷酸腺苷对突触体和可溶性纹状体酶制剂中酪氨酸羟化酶活性的刺激作用。
Brain Res. 1976 Jun 18;109(3):563-74. doi: 10.1016/0006-8993(76)90035-4.