福斯高林和霍乱毒素对分泌神经降压素的大鼠C细胞系中环磷酸腺苷释放的影响。

Effects of forskolin and cholera toxin on cyclic AMP release in a neurotensin-secreting rat C-cell line.

作者信息

Zeytin F, Mougin C, Brazeau P, Ling N

出版信息

Biochem Biophys Res Commun. 1984 Apr 30;120(2):540-7. doi: 10.1016/0006-291x(84)91288-9.

Abstract

The effects of forskolin and cholera toxin on the regulation of cAMP release were studied in a neurotensin-secreting rat C-cell line. The interaction of these agents with norepinephrine, a potent neurotensin secretagogue, was also investigated. Forskolin stimulated cAMP release 10(2)-10(3) fold while it increased neurotensin release 2-3 fold. Cholera toxin caused a 10(2)-10(3) fold increase in cAMP release and had no effect on neurotensin release. We conclude that the 44-2 C-cells provide a new model for studying the regulation of the concomitant (via forskolin) or independent (via cholera toxin) secretion of cyclic AMP and/or neurotensin.

摘要

在一种分泌神经降压素的大鼠C细胞系中研究了福斯高林和霍乱毒素对环磷酸腺苷（cAMP）释放调节的影响。还研究了这些药物与去甲肾上腺素（一种有效的神经降压素促分泌剂）的相互作用。福斯高林刺激cAMP释放增加10² - 10³倍，同时使神经降压素释放增加2 - 3倍。霍乱毒素使cAMP释放增加10² - 10³倍，而对神经降压素释放无影响。我们得出结论，44 - 2 C细胞为研究环磷酸腺苷和/或神经降压素伴随性（通过福斯高林）或独立性（通过霍乱毒素）分泌的调节提供了一个新模型。