长臂猿白血病病毒DNA聚合酶的纯化与特性分析

Purification and characterization of gibbon ape leukemia virus DNA polymerase.

作者信息

Sarin P S, Gallo R C

出版信息

Biochim Biophys Acta. 1976 Dec 1;454(2):212-21. doi: 10.1016/0005-2787(76)90225-2.

Abstract

An RNA directed DNA polymerase was purified over 2500 fold from gibbon ape leukemia virus by successive column chromatography on Sephadex G100, DEAE cellulose, phosphocellulose and hydroxyapatite. The purified DNA polymerase has a molecular weight of 68 000, a pH optimum of 7.5, a Mn2+ optimum of 0.8 mM, and KCl optimum of 80 mM. The purified enzyme transcribes heteropolymeric regions of viral 60-70 S RNA isolated from avian myeloblastosis virus, Rauscher murine leukemia virus and simian sarcoma virus and it is inhibited by antiserum prepared against either gibbon ape leukemia virus or simian sarcoma virus DNA polymerases.

摘要

通过在葡聚糖凝胶G100、二乙氨基乙基纤维素、磷酸纤维素和羟基磷灰石上连续进行柱层析，从长臂猿白血病病毒中纯化出一种RNA指导的DNA聚合酶，纯化倍数超过2500倍。纯化后的DNA聚合酶分子量为68000，最适pH为7.5，最适锰离子浓度为0.8 mM，最适氯化钾浓度为80 mM。该纯化酶能转录从禽成髓细胞瘤病毒、劳氏鼠白血病病毒和猿猴肉瘤病毒中分离出的病毒60 - 70 S RNA的异聚区域，并且被针对长臂猿白血病病毒或猿猴肉瘤病毒DNA聚合酶制备的抗血清所抑制。