通过微波功率饱和对三种铁氧化还原蛋白进行表征。
Characterization of three iron ferredoxins by microwave power saturation.
作者信息
Bertrand P, Guigliarelli B, Meyer J, Gayda J P
出版信息
Biochimie. 1984 Feb;66(2):77-9. doi: 10.1016/0300-9084(84)90182-2.
PMID:6329322
Abstract
We have measured the electronic spin lattice relaxation time T1 in the temperature range 4 K-10 K, by microwave power saturation on the 3Fe ferredoxins from Desulfovibrio gigas and Azotobacter vinelandii. The comparison with the results previously obtained on other iron sulfur proteins emphasizes the particularly fast relaxing properties of the E.P.R. signal in 3Fe ferredoxins. These results support the models of the active site which predict very low lying excited levels.
摘要
我们通过对来自巨大脱硫弧菌和棕色固氮菌的3Fe铁氧化还原蛋白进行微波功率饱和，测量了4 K至10 K温度范围内的电子自旋晶格弛豫时间T1。与先前在其他铁硫蛋白上获得的结果进行比较，突出了3Fe铁氧化还原蛋白中E.P.R.信号特别快的弛豫特性。这些结果支持了预测存在极低激发能级的活性位点模型。
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