Belardetti F, Brunelli M, Demontis G, Sonetti D
Brain Res. 1984 May 21;300(1):91-102. doi: 10.1016/0006-8993(84)91343-x.
Intracellular recordings from T mechanosensory cells of Hirudo medicinalis showed, as previously demonstrated, that repetitive firing is followed by a long-lasting hyperpolarization. Serotonin application at two concentrations (1 microM and 50 microM) depressed this hyperpolarization by up to 2/3; the effect was dose-dependent, long-lasting and reversible. Intracellular stimulation of giant serotonergic neurons (Retzius cells, Rz) mimicked serotonin perfusion: the effect was proportional to the number of spikes fired by Retzius cells. The combined use of intracellular iontophoretic injection of horseradish peroxidase and lucifer yellow indicated the possible sites of contact between Rz and T cells. The effect of serotonin, released by Rz cells, is discussed with respect to its possible physiological significance.
如先前所示，对医用水蛭的T机械感觉细胞进行的细胞内记录表明，重复放电后会出现长时间的超极化。应用两种浓度（1微摩尔和50微摩尔）的血清素可使这种超极化降低多达2/3；该效应具有剂量依赖性、持续性且可逆。对巨大的血清素能神经元（雷丘斯细胞，Rz）进行细胞内刺激可模拟血清素灌注：该效应与雷丘斯细胞发放的动作电位数量成正比。联合使用细胞内离子电渗法注射辣根过氧化物酶和荧光黄可显示Rz细胞与T细胞之间可能的接触位点。文中讨论了Rz细胞释放的血清素的效应及其可能的生理意义。
