Abecassis J, Collard R, Eber M, Pusel J, Fricker J P, Methlin G
Int J Cancer. 1984 Jun 15;33(6):821-4. doi: 10.1002/ijc.2910330617.
Proteolytic and sialyltransferase activities were determined in extracts of 65 human primary breast tumors, 6 lymph node metastases, 6 fibroadenomas and 27 normal tissues. Using proteins and synthetic selective substrates, we observed the presence of collagen-peptidases, plasminogen activator, cathepsin-B and cathepsin-D-like enzymes, and sialyltransferase. No active or trypsin-activatable type-IV collagenase activity was detected. Although individual variations between tumors were large, proteinase and sialyltransferase contents were significantly elevated in malignant breast tissues. Enzyme activities were found to be related to the epithelial volume of the tumor. No significant correlation was found between the proteinase or sialyltransferase activities and the degree of differentiation of the tumor cells, or the degree to which tumors had metastasized to regional lymph nodes. Since large variations of enzyme levels apparently reflect the heterogeneity of epithelial cell densities in tumor samples, proteolytic or sialyltransferase activities cannot therefore be used as a measure of quantitative evaluation of invasive properties in breast cancer.
在65例原发性人类乳腺肿瘤、6例淋巴结转移瘤、6例纤维腺瘤和27例正常组织的提取物中测定了蛋白水解酶和唾液酸转移酶活性。使用蛋白质和合成选择性底物，我们观察到了胶原肽酶、纤溶酶原激活剂、组织蛋白酶B和组织蛋白酶D样酶以及唾液酸转移酶的存在。未检测到活性或可被胰蛋白酶激活的IV型胶原酶活性。尽管肿瘤之间的个体差异很大，但恶性乳腺组织中的蛋白酶和唾液酸转移酶含量显著升高。发现酶活性与肿瘤的上皮体积有关。蛋白酶或唾液酸转移酶活性与肿瘤细胞的分化程度或肿瘤转移至区域淋巴结的程度之间未发现显著相关性。由于酶水平的巨大差异显然反映了肿瘤样本中上皮细胞密度的异质性，因此蛋白水解酶或唾液酸转移酶活性不能用作评估乳腺癌侵袭性的定量指标。
