Pontisso P, Falcieri E, Schiavon E, Alberti A, Realdi G
J Med Virol. 1984;13(4):355-60. doi: 10.1002/jmv.1890130406.
Receptors for polymerized human serum albumin ( pHSA ) were studied by solid-phase radioimmunoassay on different hepatitis B surface antigen (HBsAg) particles subpopulations prepared both from hepatitis B e antigen (HBeAg) and from anti-HBe-positive sera. HBsAg particles in HBeAg-positive serum showed higher expression of the receptor compared with HBsAg particles from anti-HBe-positive serum. Analysis of different morphological forms of virus particles was performed after separation by density-gradient ultracentrifugation. Maximum receptor expression was detected in HBV particles containing fractions while the 22-nm HBsAg particles had significantly lower receptor activity. These observations support the hypothesis of a pathogenetic role of the pHSA receptor in mediating virus access to hepatocytes. Indeed, the higher pHSA binding activity on HBV particles could allow selective attachment of the infectious virion to liver cells that bear similar albumin receptors on their surface.
通过固相放射免疫测定法，对从乙肝e抗原（HBeAg）和抗-HBe阳性血清制备的不同乙肝表面抗原（HBsAg）颗粒亚群上的聚合人血清白蛋白（pHSA）受体进行了研究。与抗-HBe阳性血清中的HBsAg颗粒相比，HBeAg阳性血清中的HBsAg颗粒显示出更高的受体表达。通过密度梯度超速离心分离后，对病毒颗粒的不同形态形式进行了分析。在含有HBV颗粒的组分中检测到最大受体表达，而22纳米的HBsAg颗粒的受体活性明显较低。这些观察结果支持了pHSA受体在介导病毒进入肝细胞中起致病作用的假说。事实上，HBV颗粒上较高的pHSA结合活性可以使感染性病毒粒子选择性地附着于表面带有类似白蛋白受体的肝细胞。
