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钙调蛋白和环磷酸腺苷刺激下MAP-2的差异磷酸化作用

Differential phosphorylation of MAP-2 stimulated by calcium-calmodulin and cyclic AMP.

作者信息

Schulman H

出版信息

Mol Cell Biol. 1984 Jun;4(6):1175-8. doi: 10.1128/mcb.4.6.1175-1178.1984.

DOI:10.1128/mcb.4.6.1175-1178.1984
PMID:6330535
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC368889/
Abstract

Comparison of cyclic AMP- and calcium-dependent phosphorylation in rat brain cytosol reveals MAP-2 to be a common endogenous substrate. Examination of limited protease digestion patterns indicates that the two kinases phosphorylate MAP-2 at distinct sites and suggests that such phosphorylation may have differential effects on MAP-2 function.

摘要

大鼠脑细胞溶胶中依赖环磷酸腺苷（cAMP）和钙的磷酸化作用比较显示，微管相关蛋白2（MAP-2）是一种常见的内源性底物。对有限蛋白酶消化模式的研究表明，这两种激酶在不同位点使MAP-2磷酸化，提示这种磷酸化可能对MAP-2功能产生不同影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d5/368889/8ef60b634251/molcellb00148-0183-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d5/368889/0a2f809986a0/molcellb00148-0182-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d5/368889/fc3b57dd041f/molcellb00148-0182-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d5/368889/8ef60b634251/molcellb00148-0183-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d5/368889/0a2f809986a0/molcellb00148-0182-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d5/368889/fc3b57dd041f/molcellb00148-0182-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/14d5/368889/8ef60b634251/molcellb00148-0183-a.jpg

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Differential phosphorylation of MAP-2 stimulated by calcium-calmodulin and cyclic AMP.钙调蛋白和环磷酸腺苷刺激下MAP-2的差异磷酸化作用
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引用本文的文献

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本文引用的文献

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Purification and characterization of a Ca2+- and calmodulin-dependent protein kinase from rat brain.大鼠脑中一种钙和钙调蛋白依赖性蛋白激酶的纯化与特性分析
J Neurochem. 1982 Dec;39(6):1607-17. doi: 10.1111/j.1471-4159.1982.tb07994.x.
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Modification of microtubule steady-state dynamics by phosphorylation of the microtubule-associated proteins.通过微管相关蛋白的磷酸化作用对微管稳态动力学进行修饰。
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3413-7. doi: 10.1073/pnas.78.6.3413.
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Purification and characterization of a calmodulin-dependent kinase from rat brain cytosol able to phosphorylate tubulin and microtubule-associated proteins.
从微管制剂中分离内源性钙调蛋白依赖性激酶和环磷酸腺苷依赖性激酶。
Proc Natl Acad Sci U S A. 1985 May;82(10):3202-6. doi: 10.1073/pnas.82.10.3202.
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Identification of a 52-kD calmodulin-binding protein associated with the mitotic spindle apparatus in mammalian cells.在哺乳动物细胞中鉴定出一种与有丝分裂纺锤体装置相关的52-kD钙调蛋白结合蛋白。
J Cell Biol. 1986 Nov;103(5):1855-61. doi: 10.1083/jcb.103.5.1855.
5
Activation of the multifunctional Ca2+/calmodulin-dependent protein kinase by autophosphorylation: ATP modulates production of an autonomous enzyme.通过自身磷酸化激活多功能钙/钙调蛋白依赖性蛋白激酶：ATP调节自主酶的产生。
Proc Natl Acad Sci U S A. 1986 Dec;83(24):9497-501. doi: 10.1073/pnas.83.24.9497.
6
Ca2+-dependent phosphorylation of tyrosine hydroxylase in PC12 cells.PC12细胞中酪氨酸羟化酶的钙离子依赖性磷酸化
J Cell Biol. 1985 Oct;101(4):1182-90. doi: 10.1083/jcb.101.4.1182.
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Differential vulnerability of microtubule components in cerebral ischemia.脑缺血中微管成分的差异易损性
Acta Neuropathol. 1990;80(5):499-505. doi: 10.1007/BF00294610.
8
Phosphorylation determines the binding of microtubule-associated protein 2 (MAP2) to microtubules in living cells.磷酸化作用决定了微管相关蛋白2（MAP2）在活细胞中与微管的结合。
J Cell Biol. 1991 Aug;114(4):735-43. doi: 10.1083/jcb.114.4.735.
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J Biol Chem. 1983 Oct 25;258(20):12632-40.
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Extensive cAMP-dependent and cAMP-independent phosphorylation of microtubule-associated protein 2.
J Biol Chem. 1983 Jun 25;258(12):7883-6.
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Phosphorylation of microtubule-associated proteins regulates their interaction with actin filaments.微管相关蛋白的磷酸化作用调节其与肌动蛋白丝的相互作用。
J Biol Chem. 1983 Jun 10;258(11):7064-71.
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Disassembly of microtubules by the action of calmodulin-dependent protein kinase (Kinase II) which occurs only in the brain tissues.
Biochem Biophys Res Commun. 1983 Jan 14;110(1):287-91. doi: 10.1016/0006-291x(83)91293-7.
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Structure and phosphorylation of microtubule-associated protein 2 (MAP 2).微管相关蛋白2（MAP 2）的结构与磷酸化
Proc Natl Acad Sci U S A. 1980 Jun;77(6):3206-10. doi: 10.1073/pnas.77.6.3206.
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A dynein-like protein from brain.
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A dynein-like protein associated with neurotubules.一种与神经微管相关的动力蛋白样蛋白。
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