Carstens M E, Neethling A C, Taljaard J J
Neurochem Res. 1984 Feb;9(2):195-206. doi: 10.1007/BF00964167.
During phosphorylation of acetylcholine receptor (AChR)-enriched membrane preparations from Torpedo fuscomaculata , phosphate is incorporated into a single protein, with a molecular weight corresponding to that of one of the receptor subunits (37,000 daltons). This protein also seems to contain the receptor binding site. ATP binds to four protein species, one of which corresponds to a different subunit of the receptor (molecular weight 45,000). Phosphorylation of these membrane preparations is affected by several factors, known to be involved in postsynaptic events. Ca2+ (10 microM) inhibits the reaction, whereas cGMP (20 microM), causes stimulation. Furthermore it has been shown that the agonists, acetylcholine, and carbamylcholine (10 microM and 1 microM) stimulate the phosphorylation reaction, while the antagonists, tubocurarine, hexamethonium, and decamethonium (1 microM), cause inhibition.
在对来自斑纹电鳐富含乙酰胆碱受体（AChR）的膜制剂进行磷酸化过程中，磷酸被掺入一种单一蛋白质中，其分子量与受体亚基之一（37,000道尔顿）相对应。这种蛋白质似乎也包含受体结合位点。ATP与四种蛋白质结合，其中一种对应于受体的不同亚基（分子量45,000）。这些膜制剂的磷酸化受几个已知参与突触后事件的因素影响。Ca2 +（10微摩尔）抑制该反应，而cGMP（20微摩尔）则起刺激作用。此外，已经表明激动剂乙酰胆碱和氨甲酰胆碱（10微摩尔和1微摩尔）刺激磷酸化反应，而拮抗剂筒箭毒碱、六甲铵和十甲铵（1微摩尔）则导致抑制。
