兔皮质集合管顶端膜钙激活钾通道的单通道记录
Single channel recordings of calcium-activated potassium channels in the apical membrane of rabbit cortical collecting tubules.
作者信息
Hunter M, Lopes A G, Boulpaep E L, Giebisch G H
出版信息
Proc Natl Acad Sci U S A. 1984 Jul;81(13):4237-9. doi: 10.1073/pnas.81.13.4237.
Abstract
Recordings of single potassium channels from the apical membrane of rabbit cortical collecting tubule have been achieved using the patch-clamp technique. The conductive properties of the channel have been studied in inside-out patches. The slope conductance of the open channel is approximately equal to 90 pS. The channel is selective to potassium over sodium, with a selectivity ratio of 9:1. Decreasing the calcium concentration of the solution bathing the cytoplasmic face of the patch results in a decrease of the open-channel probability. Decreasing the calcium concentration to 10 nM or less completely inhibited channel activity. The channel is also inhibited by barium in a dose-dependent fashion.
摘要
利用膜片钳技术已实现了对兔皮质集合管顶端膜上单个钾通道的记录。已在向外膜片模式下研究了该通道的导电特性。开放通道的斜率电导约为90皮西门子。该通道对钾的选择性高于钠，选择性比率为9:1。降低灌流膜片胞质面溶液的钙浓度会导致开放通道概率降低。将钙浓度降至10纳摩尔或更低会完全抑制通道活性。该通道也会被钡以剂量依赖方式抑制。
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