Furesz J, Armstrong R E, Contreras G, Wachmann B
Rev Infect Dis. 1984 May-Jun;6 Suppl 2:S528-34.
From 1962 to 1981, 629 poliovirus strains of human and sewage origin were assayed for genetic markers in the kinetic serum neutralization ( KSN ) and temperature marker (rct) tests. The KSN test proved to be most dependable: 97% of strains tested could be classified as either vaccine-like (VL) or non-vaccine-like ( NVL ). All but two of the 18 virus strains whose classification was inconclusive in the KSN test were further characterized by micro serum neutralization assay. The correlation between the results of the rct and KSN marker tests was not satisfactory for the characterization of poliovirus types 1 and 3. Numerous type 1 virus strains were analyzed with strain-specific monoclonal antibodies for antigenic markers and were examined in polyacrylamide gel electrophoreses for viral proteins. Whereas greater than 90% of poliovirus types 2 and 3 were found to be VL in the KSN test, 40% of type 1 isolates were classified as NVL . The latter were detected not only in the epidemics of 1962 and 1978-1979 caused by type 1 poliovirus but also were present in endemic form in the late 1960s and 1970s in various provinces. In contrast, no NVL type 2 strains were detected in any specimen, and the last type 3 NVL strains were demonstrated in 1963-1964. Thus only type 1 poliovirus appears to be endemic in Canada.
1962年至1981年期间，对629株源自人类和污水的脊髓灰质炎病毒毒株进行了动力学血清中和（KSN）试验和温度标记（rct）试验，以检测其遗传标记。结果证明KSN试验最为可靠：97%的受试毒株可被归类为疫苗样（VL）或非疫苗样（NVL）。在KSN试验中分类不确定的18株病毒毒株中，除两株外，其余均通过微量血清中和试验作了进一步鉴定。对于1型和3型脊髓灰质炎病毒的鉴定而言，rct试验结果与KSN标记试验结果之间的相关性并不令人满意。利用型特异性单克隆抗体对众多1型病毒毒株进行了抗原标记分析，并通过聚丙烯酰胺凝胶电泳对病毒蛋白进行了检测。在KSN试验中，超过90%的2型和3型脊髓灰质炎病毒被发现为VL型，而40%的1型分离株被归类为NVL型。后者不仅在1962年以及1978 - 1979年由1型脊髓灰质炎病毒引起的疫情中被检测到，而且在20世纪60年代末和70年代以地方流行形式存在于各省。相比之下，在任何标本中均未检测到NVL 2型毒株，最后一批3型NVL毒株是在1963 - 1964年发现的。因此，似乎只有1型脊髓灰质炎病毒在加拿大呈地方流行。
