Wojdani A, Attarzadeh M, Wolde-Tsadik G, Alfred L J
Toxicology. 1984 Jun;31(3-4):181-9. doi: 10.1016/0300-483x(84)90100-8.
The in vivo effects of 3 polycyclic aromatic hydrocarbons (PAH): 3-methylcholanthrene (MCA), benzo[a]pyrene (BaP) and benzo[e]pyrene (BeP) on the ability of mouse lymphocytes to bind and kill target tumor cells in vitro were measured. C57 and C3H inbred mice were preimmunized with P815 tumor cells and then treated with a single i.p. injection of corn oil alone or with varying doses of the above PAH compounds (0.5-50 mg/kg body wt). At different post-injection times, antigen sensitized splenic lymphocytes (SL) and peritoneal exudate lymphocytes (PEL) were measured for binding and killing rates, using a single cell assay. MCA doses of 5 and 50 mg/kg inhibited SL: target cell binding 29-42% and PEL: target cell binding 23-60%. BaP had a similar significant dose dependent suppression on SL and PEL binding. Target cell killing rates by SL and PEL from MCA and BaP treated C57 and C3H mice were consistently suppressed at significant levels, compared to oil injected controls (P less than 0.05). On the other hand, binding and killing rates by SL and PEL from BeP treated mice showed an inconsistent and borderline significance at the above dose levels. When measured as a function of post-injection time of MCA, binding rates of SL from both mouse strains remained essentially unchanged after 10, 30 and 45 days. Target cell killing by SL from C3H and C57 mice, however, was suppressed 55-65% after 10-30 days post-injection. At 45 days post-injection, the capacity of SL to kill target tumor cells was restored to 64-70% of control values. The results suggest that binding is an early event that depends on dose, whereas target cell killing is a function of dose and post-injection time.
测定了3种多环芳烃（PAH）：3-甲基胆蒽（MCA）、苯并[a]芘（BaP）和苯并[e]芘（BeP）在体内对小鼠淋巴细胞体外结合和杀伤靶肿瘤细胞能力的影响。将C57和C3H近交系小鼠用P815肿瘤细胞预先免疫，然后腹腔内单次注射单独的玉米油或不同剂量的上述PAH化合物（0.5 - 50 mg/kg体重）。在注射后的不同时间，使用单细胞测定法测量抗原致敏的脾淋巴细胞（SL）和腹腔渗出淋巴细胞（PEL）的结合率和杀伤率。5和50 mg/kg剂量的MCA抑制SL与靶细胞的结合29% - 42%，抑制PEL与靶细胞的结合23% - 60%。BaP对SL和PEL的结合也有类似的显著剂量依赖性抑制作用。与注射油的对照组相比，来自MCA和BaP处理的C57和C3H小鼠的SL和PEL对靶细胞的杀伤率持续受到显著抑制（P小于0.05）。另一方面，来自BeP处理小鼠的SL和PEL的结合率和杀伤率在上述剂量水平上表现出不一致且临界显著。当作为MCA注射后时间的函数进行测量时，两种小鼠品系的SL结合率在10、30和45天后基本保持不变。然而，注射后10 - 30天，C3H和C57小鼠的SL对靶细胞的杀伤被抑制55% - 65%。注射后45天，SL杀伤靶肿瘤细胞的能力恢复到对照值的64% - 70%。结果表明，结合是一个早期事件，取决于剂量，而靶细胞杀伤是剂量和注射后时间的函数。
