Kasakov L
Acta Physiol Pharmacol Bulg. 1984;10(1):13-20.
The amplitude of atropine-resistant twich contractions of muscle strips of the guinea-pig urinary bladder was not changed after 90 min continuous electrical stimulation in the presence of 100 microM hemicholinium-3, as compared with the amplitude of the corresponding contractions in control experiments without hemicholinium-3. Addition of 100 microM choline to the medium did not change the amplitude of contractions either. Methionine-enkephalin (1 microM) known as a potent inhibitor of acetylcholine release in autonomic nerves did not change the amplitude of the twich responses. Apamin (1 microM) which inhibited the non-adrenergic, non-cholinergic responses in taenia coli did not change the amplitude of the atropine-resistant contractile responses of the guinea-pig urinary bladder. It is concluded that acetylcholine is not the principle excitatory neurotransmitter in the guinea-pig urinary bladder, although some cholinergic contribution to the twich response exists. The nonadrenergic, non-cholinergic atropine-resistant contractile response of the guinea-pig urinary bladder is apamin-resistant.
在存在100微摩尔半胱氨酰胆碱-3的情况下，对豚鼠膀胱肌条进行90分钟连续电刺激后，其对阿托品耐药的抽搐收缩幅度与无半胱氨酰胆碱-3的对照实验中相应收缩的幅度相比没有变化。向培养基中添加100微摩尔胆碱也未改变收缩幅度。甲硫氨酸脑啡肽（1微摩尔）作为自主神经中乙酰胆碱释放的有效抑制剂，并未改变抽搐反应的幅度。抑制结肠带非肾上腺素能、非胆碱能反应的蜂毒明肽（1微摩尔）未改变豚鼠膀胱对阿托品耐药的收缩反应幅度。结论是，尽管胆碱能对抽搐反应有一定贡献，但乙酰胆碱不是豚鼠膀胱中的主要兴奋性神经递质。豚鼠膀胱的非肾上腺素能、非胆碱能阿托品耐药收缩反应对蜂毒明肽耐药。
