Lydic R, McCarley R W, Hobson J A
Am J Physiol. 1984 Jul;247(1 Pt 2):R135-45. doi: 10.1152/ajpregu.1984.247.1.R135.
Single-cell activity recorded from the brain stem dorsal raphe nucleus (DRN) of cats showed a regular firing pattern during wakefulness (2-3 spikes/s), decreased activity during slow-wave or synchronized sleep (1 spike/s), and cessation of regular discharge during desynchronized sleep (0.05 spikes/s). DRN discharge was negatively correlated with the onset and maintenance of the polycyclic desynchronized sleep rhythm. These findings were derived from analyses of DRN firing rate sampled during different behavioral states. The present time-course analyses of DRN discharge described for the first time the period, amplitude, and phase characteristics of DRN activity relative to the timing of behavioral states that comprise the complete sleep cycle. The time course of both DRN discharge and the occurrence of wakefulness, slow-wave (S) sleep, transition, and desynchronized (D) sleep were behaviorally manipulated. Forced locomotor activity imposed by a treadmill task shortened the period length of the sleep cycle and the duration of the DRN discharge cycle. The magnitude and direction of these shifts in period length were consistent across multiple sleep cycles, and there was a high degree of coherence between the period length of the sleep cycle and the DRN discharge profiles. The DRN discharge rate also displayed phase dependence within S and D sleep. These results support the hypothesis that the DRN has a physiological role in regulating the timing of the ultradian sleep cycle.
从猫的脑干中缝背核（DRN）记录的单细胞活动显示，在清醒状态下有规律的放电模式（2 - 3次/秒），在慢波或同步睡眠期间活动减少（1次/秒），在去同步睡眠期间规律放电停止（0.05次/秒）。DRN放电与多周期去同步睡眠节律的开始和维持呈负相关。这些发现来自于对不同行为状态下DRN放电率的分析。目前对DRN放电的时程分析首次描述了DRN活动相对于构成完整睡眠周期的行为状态时间的周期、幅度和相位特征。DRN放电的时程以及清醒、慢波（S）睡眠、过渡和去同步（D）睡眠的发生都受到行为操纵。跑步机任务施加的强制运动活动缩短了睡眠周期的时长和DRN放电周期的持续时间。这些周期长度变化的幅度和方向在多个睡眠周期中是一致的，并且睡眠周期的时长与DRN放电曲线之间存在高度的一致性。DRN放电率在S和D睡眠中也表现出相位依赖性。这些结果支持了DRN在调节超日睡眠周期时间方面具有生理作用的假设。
