Winston G W, Reitz R C
Alcohol Clin Exp Res. 1984 May-Jun;8(3):277-82. doi: 10.1111/j.1530-0277.1984.tb05511.x.
The effects of chronic ethanol ingestion on the interconversion of the active to the inactive form of glycogen phosphorylase by phosphorylase phosphatase was studied. Male and female rats also were compared. Chronic ethanol feeding decreased phosphorylase a and total phosphorylase activity in male rats. In females, no change was observed in phosphorylase a, whereas total phosphorylase activity was increased 73%. This was found to correlate with the relative activities of phosphorylase phosphatase. The data show differences between the two sexes with regard to the AMP inhibition of phosphorylase phosphatase and the caffeine stimulation of the phosphatase. Ethanol markedly enhanced the AMP inhibition of the phosphatase in males but had no effect in females. Further studies in females showed that ethanol completely obliterated the well documented stimulation of the phosphatase by caffeine; however, it did not alter the caffeine effect in males. These data suggest possible alterations in the tertiary structure of phosphorylase a.
研究了长期摄入乙醇对磷酸化酶磷酸酶将糖原磷酸化酶的活性形式转化为非活性形式的相互转化的影响。还比较了雄性和雌性大鼠。长期喂食乙醇会降低雄性大鼠的磷酸化酶a和总磷酸化酶活性。在雌性大鼠中，未观察到磷酸化酶a有变化，而总磷酸化酶活性增加了73%。发现这与磷酸化酶磷酸酶的相对活性相关。数据显示，在磷酸化酶磷酸酶的AMP抑制和磷酸酶的咖啡因刺激方面，两性之间存在差异。乙醇显著增强了雄性大鼠中磷酸酶的AMP抑制作用，但对雌性大鼠没有影响。对雌性大鼠的进一步研究表明，乙醇完全消除了咖啡因对磷酸酶的显著刺激作用；然而，它并没有改变咖啡因对雄性大鼠的作用。这些数据表明磷酸化酶a的三级结构可能发生了改变。
