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[化学感应结构中钙调蛋白的存在、纯化及其含量]

[The presence of calmodulin in chemosensory structures, its purification and content].

作者信息

Ostretsova I B, Suvorov S A, Levko A V, Etingof R N

出版信息

Biokhimiia. 1984 May;49(5):777-80.

PMID:6331533
Abstract

Chemosensory tissues, bovine olfactory epithelium and barbel of dwarf sheat-fish (Ictalurus nebulosus) rich in gustatory buds were shown to contain calmodulin. The fraction of thermostable proteins which activate brain phosphodiesterase of cyclic nucleotides was purified to homogeneity by stepwise ion-exchange chromatography on DEAE-cellulose and gel filtration on Sephacryl S-300. Some properties of calmodulin from chemosensory tissues (e. g., content, molecular weight, electrophoretic mobility, degree of activation of phosphodiesterase) are similar to those of brain calmodulin.

摘要

化学感觉组织,即富含味蕾的牛嗅觉上皮和矮胖鲶鱼(斑点叉尾鮰)的触须,被证明含有钙调蛋白。通过在DEAE-纤维素上进行分步离子交换色谱和在Sephacryl S-300上进行凝胶过滤,将激活环核苷酸脑磷酸二酯酶的热稳定蛋白部分纯化至同质。来自化学感觉组织的钙调蛋白的一些特性(例如,含量、分子量、电泳迁移率、磷酸二酯酶的激活程度)与脑钙调蛋白的特性相似。

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