微粒体介导的苯并[a]芘和3,3-二甲基-1-苯基三氮烯对大鼠肝脏起始转移核糖核酸氨酰化的刺激作用。

Microsome-mediated stimulation of the aminoacylation of rat liver initiator transfer ribonucleic acid by benzo[alpha]pyrene and 3,3-dimethyl-1-phenyltriazene.

作者信息

Hradec J, Kolar G F

出版信息

Cancer Lett. 1984 May;23(1):115-20. doi: 10.1016/0304-3835(84)90070-3.

Abstract

The charging of initiator tRNAmetF with L-methionine was significantly stimulated by pre-incubation of unfractionated tRNA from rat liver with benzo [alpha]pyrne (BP) or 3,3-dimethyl-1-phenyltriazine (DMPT). The presence of microsomal enzymes from rat liver and of NADPH was absolutely required for this effect. Maximum enhancement was obtained after 60 min of incubation with 10(-4)-10(-8) mumol/ml of either compound tested. It appears that either proximate carcinogen, BP or DMPT, must be converted by microsomal enzymes into its direct-acting metabolites (ultimate carcinogens) which interact with initiator tRNA and thereby specifically modulate its aminoacylation.

摘要

用苯并[a]芘（BP）或3,3 - 二甲基 - 1 - 苯基三嗪（DMPT）对大鼠肝脏的未分级tRNA进行预孵育，可显著刺激起始tRNAmetF与L - 甲硫氨酸的充电反应。这种效应绝对需要大鼠肝脏微粒体酶和NADPH的存在。用10^(-4) - 10^(-8) μmol/ml的任何一种受试化合物孵育60分钟后可获得最大增强效果。似乎任何一种近致癌物，BP或DMPT，都必须被微粒体酶转化为其直接作用的代谢物（最终致癌物），这些代谢物与起始tRNA相互作用，从而特异性地调节其氨酰化作用。