Arnot D, Lillie J W, Auffray C, Kappes D, Strominger J L
Immunogenetics. 1984;20(3):237-52. doi: 10.1007/BF00364206.
We have constructed cDNA clone libraries from two lymphoblastoid cell lines, JY (HLA-A2, B7, C untypeable) and LB (HLA-A28, B40, Cw3), and isolated clones encoding class I HLA antigens. We have characterized short oligonucleotide probes derived from the coding region of the HLA class I antigens which are specific for the HLA-A and -B loci. These probes have been used to subdivide the class I cDNA clones into subclasses. DNA sequencing of several HLA-A and -B related clones has allowed us to extend the primary structural characterization of these cell-surface antigens. This analysis has also detected a sequence polymorphism at the HLA-A locus, indicating that the previously considered homozygous typing cell line LB expresses two alleles of similar, although not identical, serological specificity.
我们从两个淋巴母细胞系JY（HLA - A2、B7、C型无法分型）和LB（HLA - A28、B40、Cw3）构建了cDNA克隆文库，并分离出编码I类HLA抗原的克隆。我们对源自HLA I类抗原编码区的短寡核苷酸探针进行了表征，这些探针对于HLA - A和 - B位点具有特异性。这些探针已用于将I类cDNA克隆细分为亚类。对几个与HLA - A和 - B相关的克隆进行DNA测序，使我们能够扩展这些细胞表面抗原的一级结构表征。该分析还在HLA - A位点检测到序列多态性，表明先前认为是纯合分型细胞系的LB表达了两个血清学特异性相似但不相同的等位基因。
