Siwarski D F, Barra Y, Jay G, Rogers M J
Immunogenetics. 1985;21(3):267-76. doi: 10.1007/BF00375379.
There is unequivocal evidence that a relatively nonpolymorphic class I gene (designated Q10) from the Qa region of inbred mice encodes a secreted class I molecule. We have used a cDNA probe specific for this gene and an antiserum specific for its secreted protein product to investigate the occurrence and expression of this gene in different species of wild mice broadly representing the entire genus Mus. Evidence is presented that a Q10-like gene has been conserved and is transcribed and translated throughout the genus, suggesting that it serves an important function. However, the data also show that some differences have appeared in this gene over the period of evolutionary time covered by this sampling of wild mice. These results indicate that a specific class I DNA probe isolated from inbred mice can be used to discriminate a particular gene among the multiple class I genes present in other species.
有明确证据表明，近交系小鼠Qa区域中一个相对非多态性的I类基因（命名为Q10）编码一种分泌型I类分子。我们使用了针对该基因的cDNA探针和针对其分泌蛋白产物的抗血清，来研究这个基因在广泛代表整个小家鼠属的不同野生小鼠物种中的出现情况和表达。有证据表明，一个类似Q10的基因已被保留下来，并且在整个属中都进行转录和翻译，这表明它发挥着重要功能。然而，数据也显示，在本次野生小鼠采样所涵盖的进化时间段内，这个基因出现了一些差异。这些结果表明，从近交系小鼠中分离出的特定I类DNA探针可用于在其他物种中存在的多个I类基因中区分出一个特定基因。
