Stadecker M J, Ebner S A
J Immunol. 1984 Oct;133(4):2231-6.
Macrophages from schistosomal egg granulomas of athymic mice (nu/nu GM) and their euthymic littermates (nu/+ GM) were analyzed phenotypically for the expression of antigens encoded by the I-A subregion of the major histocompatibility complex and for their ability to perform as antigen-presenting cells. Only 11 to 15% of nu/nu GM expressed I-A antigens as compared to 61.5 to 75% of nu/+ GM. Although both populations of cells appeared to be equally effective as antigen-presenting cells appropriately sensitized lymphocytes in the presence of specific antigens--soluble schistosomal egg antigen (SEA) and human gamma-globulin (HGG)--only nu/nu GM, but not nu/+ GM, were found to stimulate I-A-restricted proliferation of schistosome-sensitized T cell populations in the absence of SEA added in vitro. Furthermore, nu/nu GM but not nu/+ GM were shown to exhibit significant proliferative capacity in vitro, but this phenomenon could not account for the observed difference in SEA-independent T cell stimulation. Finally, culture supernatants from nu/nu GM displayed significant thymocyte-stimulating activity, consistent with interleukin 1, which was not observed in nu/+ GM. These findings point to significant differences between nu/nu GM and nu/+ GM, which may be part of an adaptive mechanism of granulomatous reactivity in the absence of a competent T cell system.
对无胸腺小鼠（nu/nu GM）及其有胸腺的同窝小鼠（nu/+ GM）血吸虫卵肉芽肿中的巨噬细胞进行了表型分析，以检测主要组织相容性复合体I-A亚区编码抗原的表达情况及其作为抗原呈递细胞的能力。与61.5%至75%的nu/+ GM相比，只有11%至15%的nu/nu GM表达I-A抗原。尽管在存在特异性抗原——可溶性血吸虫卵抗原（SEA）和人γ球蛋白（HGG）的情况下，这两种细胞群体作为抗原呈递细胞对适当致敏的淋巴细胞似乎同样有效，但在体外未添加SEA的情况下，仅发现nu/nu GM，而非nu/+ GM，能刺激血吸虫致敏T细胞群体的I-A限制性增殖。此外，nu/nu GM而非nu/+ GM在体外显示出显著的增殖能力，但这种现象无法解释在不依赖SEA的T细胞刺激中观察到的差异。最后，nu/nu GM的培养上清液显示出显著的胸腺细胞刺激活性，与白细胞介素1一致，而在nu/+ GM中未观察到这种情况。这些发现表明nu/nu GM和nu/+ GM之间存在显著差异，这可能是在缺乏正常T细胞系统时肉芽肿反应性适应性机制的一部分。
