Johannessen A C, Nilsen R, Skaug N
Scand J Dent Res. 1984 Aug;92(4):325-33. doi: 10.1111/j.1600-0722.1984.tb00899.x.
Cell infiltrates in sections of paraffin-embedded tissue of 36 human periapical inflammatory lesions (15 granulomas and 21 cysts) were examined by staining for acid alpha-naphthyl acetate esterase (ANAE). Thirty-four of the lesions showed ANAE reactive T lymphocytes, plasma cells and cells of the monocyte/macrophage cell line. Large granular lymphocytes (NK cells) could not be identified with certainty among the ANAE positive cells. Two specimens were free of ANAE reactive cells. Differential cell counts on sections from 15 lesions (7 granulomas and 8 cysts) showed that T lymphocytes were the predominating ANAE reactive cells (23%), followed by plasma cells (15%) and monocytes/macrophages (11%). 51% of the inflammatory cells were ANAE negative. There was no marked difference between the granulomas and the cysts. Inhibition with NaF (10-100 mM) gave a gradual reduction in number of ANAE positive cells, some macrophages being the only cells resistant to 100 mM NaF. The results suggest that both humoral and cellular immune reactions are likely to occur in periapical inflammatory lesions.
通过酸性α-萘乙酸酯酶（ANAE）染色，对36例人类根尖周炎性病变（15例肉芽肿和21例囊肿）石蜡包埋组织切片中的细胞浸润情况进行了检查。34例病变显示有ANAE反应性T淋巴细胞、浆细胞和单核细胞/巨噬细胞系细胞。在ANAE阳性细胞中不能确切识别出大颗粒淋巴细胞（NK细胞）。有2个标本没有ANAE反应性细胞。对15例病变（7例肉芽肿和8例囊肿）切片进行的细胞分类计数显示，T淋巴细胞是主要的ANAE反应性细胞（23%），其次是浆细胞（15%）和单核细胞/巨噬细胞（11%）。51%的炎性细胞ANAE阴性。肉芽肿和囊肿之间没有明显差异。用NaF（10 - 100 mM）抑制后，ANAE阳性细胞数量逐渐减少，一些巨噬细胞是唯一对100 mM NaF有抗性的细胞。结果表明，根尖周炎性病变中可能同时发生体液免疫和细胞免疫反应。
