Eldridge J H, Yaffe L J, Ryan J J, Kiyono H, Scher I, McGhee J R
J Immunol. 1984 Nov;133(5):2308-11.
Treatment of the Peyer's patch (PP) B cells from X-linked immunodeficient (xid) (CBA/N X DBA/2)F1 male mice with anti-Lyb-5 plus complement kills approximately 75% of these cells, although xid spleen B cells are unaffected. Cytotoxic depletion of Lyb-5+ cells renders the xid PP B cell population unable to generate an in vitro direct plaque-forming cell response to the TI-2 antigen TNP-Ficoll. In addition, the B cell-enriched population from the PP of xid (CBA/N X CBA/J)F1 male mice were strong stimulators of proliferation in an M1s-defined MLR, thereby demonstrating that they also express the M1s antigen(s). Two cell surface antigens associated with mature B cells are therefore expressed by xid PP B cells, suggesting that the TNP-Ficoll responsive cells in this population are mature B cell.
用抗Lyb-5加补体处理X连锁免疫缺陷（xid）（CBA/N×DBA/2）F1雄性小鼠的派尔集合淋巴结（PP）B细胞，约75%的这些细胞会被杀死，尽管xid脾脏B细胞不受影响。Lyb-5+细胞的细胞毒性清除使xid PP B细胞群体无法对TI-2抗原TNP-菲可产生体外直接空斑形成细胞反应。此外，来自xid（CBA/N×CBA/J）F1雄性小鼠PP的富含B细胞的群体在M1s定义的混合淋巴细胞反应（MLR）中是强烈的增殖刺激物，从而表明它们也表达M1s抗原。因此，xid PP B细胞表达两种与成熟B细胞相关的细胞表面抗原，这表明该群体中对TNP-菲可反应的细胞是成熟B细胞。
