Montesano R, Mossaz A, Ryser J E, Orci L, Vassalli P
J Cell Biol. 1984 Nov;99(5):1706-15. doi: 10.1083/jcb.99.5.1706.
We report here that interleukins have a dramatic effect on extracellular matrix production by cultured endothelial cells. Human umbilical vein endothelial cells incubated with growth media conditioned by lectin-activated human peripheral blood mononuclear leukocytes undergo marked changes in cell shape and elaborate a highly organized extracellular material that is not detectable in untreated cultures. This material has the following characteristics: (a) it is not recognizable by electron microscopy unless the cationic dye, Alcian blue, is added to the fixative; (b) it is visualized as a network of branching and anastomosing fibrils of various thickness that can be resolved into bundles of fine filaments; (c) it is associated with the cell surface, extends between contiguous cells, and coats the culture substrate; (d) it is removed by digestion with glycosaminoglycan-degrading enzymes, such as crude heparinase and chondroitinase ABC. These results demonstrate that soluble factors released by activated peripheral blood mononuclear leukocytes (interleukins) stimulate cultured human umbilical vein endothelial cells to produce a highly structured pericellular matrix containing glycosaminoglycans (probably chondroitin sulfate and/or hyaluronic acid) as a major constituent. We speculate that this phenomenon corresponds to an early step of angiogenesis as observed in vivo as a consequence of interleukin release.
我们在此报告，白细胞介素对培养的内皮细胞产生细胞外基质具有显著影响。用人外周血单核白细胞经凝集素激活后所产生的生长培养基培养人脐静脉内皮细胞，细胞形态会发生显著变化，并形成一种高度有序的细胞外物质，而在未经处理的培养物中则检测不到这种物质。这种物质具有以下特征：（a）除非在固定剂中加入阳离子染料阿尔辛蓝，否则在电子显微镜下无法识别；（b）它表现为一个由不同厚度的分支和吻合纤维组成的网络，这些纤维可分解为细丝束；（c）它与细胞表面相关，在相邻细胞之间延伸，并覆盖培养底物；（d）用糖胺聚糖降解酶，如粗制肝素酶和软骨素酶ABC消化可将其去除。这些结果表明，活化的外周血单核白细胞释放的可溶性因子（白细胞介素）刺激培养的人脐静脉内皮细胞产生一种高度结构化的细胞周围基质，其主要成分是糖胺聚糖（可能是硫酸软骨素和/或透明质酸）。我们推测，这种现象对应于体内观察到的由于白细胞介素释放而导致的血管生成的早期步骤。
