Flynn A
J Nutr. 1984 Nov;114(11):2034-42. doi: 10.1093/jn/114.11.2034.
Plant lectin-induced proliferation of lymphocytes in vitro in both whole-spleen cell and T cell-enriched cultures was markedly effected by depletion of media copper, magnesium or zinc. The T lymphocyte-oriented mitogens concanavalin A and phytohemagglutinin and the B lymphocyte-oriented mitogen lipopolysaccharide were used to study variations in [3H]thymidine incorporation in lymphocytes cultured in media deficient in one mineral element. Since the stimulatory action of these mitogens also relates to the interaction of lymphocytes with accessory cells, we looked at the phagocytic ability of accessory cells cultured in the depleted media. In addition, we determined the variations in cell surface markers for B lymphocytes (Ia), T lymphocytes (Thy 1.2, Lyt 1 and Lyt 2) and accessory cells (Ia). Media depleted of copper, magnesium or zinc did not support normal T-lymphocyte proliferation but did support normal B-lymphocyte proliferation. The phagocytic ability of magnesium-deficient and zinc-deficient accessory cells was also depressed. This was related to depressed Ia expressions in the magnesium-deficient and zinc-deficient whole-spleen cell cultures. Total T-lymphocyte numbers, as well as Lyt 1+ cell percentages, were unchanged by media depletion, whereas Lyt 2+ cell percentages were depressed in both copper-deficient and magnesium-deficient splenocyte cultures.
植物凝集素诱导的全脾细胞和富含T细胞的培养物中淋巴细胞在体外的增殖，受到培养基中铜、镁或锌缺乏的显著影响。使用T淋巴细胞定向有丝分裂原刀豆球蛋白A和植物血凝素以及B淋巴细胞定向有丝分裂原脂多糖，来研究在缺乏一种矿物质元素的培养基中培养的淋巴细胞中[3H]胸苷掺入的变化。由于这些有丝分裂原的刺激作用也与淋巴细胞和辅助细胞的相互作用有关，我们研究了在缺乏矿物质的培养基中培养的辅助细胞的吞噬能力。此外，我们测定了B淋巴细胞（Ia）、T淋巴细胞（Thy 1.2、Lyt 1和Lyt 2）以及辅助细胞（Ia）的细胞表面标志物的变化。缺乏铜、镁或锌的培养基不支持正常的T淋巴细胞增殖，但支持正常的B淋巴细胞增殖。缺乏镁和锌的辅助细胞的吞噬能力也受到抑制。这与缺乏镁和锌的全脾细胞培养物中Ia表达的降低有关。培养基缺乏对总T淋巴细胞数量以及Lyt 1 +细胞百分比没有影响，而在缺乏铜和镁的脾细胞培养物中Lyt 2 +细胞百分比降低。
