噬菌体P1 lox-Cre系统的位点特异性重组。Cre介导的两个lox位点的联会。
Site-specific recombination by the bacteriophage P1 lox-Cre system. Cre-mediated synapsis of two lox sites.
作者信息
Hamilton D L, Abremski K
出版信息
J Mol Biol. 1984 Sep 15;178(2):481-6. doi: 10.1016/0022-2836(84)90154-2.
PMID:6333513
Abstract
The bacteriophage P1-encoded recombinase Cre forms a simple DNA-protein complex at the specific recognition site loxP. Furthermore, Cre is able to mediate a synaptic union of two loxP sites. When two loxP sites are on the same linear DNA molecule, Cre binds the two sites together to form a circular protein-DNA complex. These complexes can be resolved into a linear DNA molecule and a closed circular DNA molecule, the end products of site-specific recombination.
摘要
噬菌体P1编码的重组酶Cre在特定识别位点loxP处形成简单的DNA-蛋白质复合物。此外，Cre能够介导两个loxP位点的突触结合。当两个loxP位点位于同一线性DNA分子上时，Cre将这两个位点结合在一起，形成环状蛋白质-DNA复合物。这些复合物可分解为线性DNA分子和闭环DNA分子，即位点特异性重组的终产物。
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