Nishimura T, Kozutsumi H, Hashimoto Y
Thymus. 1984;6(4):225-33.
Interleukin 2 (IL 2) was obtained from culture fluids of Con A-stimulated rat spleen cells and purified by repeated Sephacryl S-200 column chromatography. The purified IL 2 showed a direct mitogenic effect on C57BL/6 mouse spleen cells and lymph node cells but not on unfractionated thymocytes. Contamination of Con A in the IL 2 was ruled out by the finding that the mitogenic effect of the IL 2 was not diminished by addition of alpha-methyl-D-mannoside in the reaction mixture. The treatment of the spleen cells with anti-Thy 1.2 and complement abolished the response to the IL 2, indicating that the IL 2-responding cells bear a T cell marker. Although unfractionated thymocytes did not blastogenically respond to the IL 2, fractionation of thymocytes by means of discontinuous gradient centrifugation with Percoll resulted in a minor cell population which strongly responded to the IL 2 stimulation. The cells were distributed in lower density fractions. The cells distributed in higher density fractions did not respond to the IL 2.
白细胞介素2（IL-2）从刀豆蛋白A刺激的大鼠脾细胞培养液中获得，并通过反复的Sephacryl S-200柱层析进行纯化。纯化后的IL-2对C57BL/6小鼠脾细胞和淋巴结细胞具有直接促有丝分裂作用，但对未分级的胸腺细胞无此作用。通过发现在反应混合物中添加α-甲基-D-甘露糖苷不会减弱IL-2的促有丝分裂作用，排除了IL-2中刀豆蛋白A的污染。用抗Thy 1.2和补体处理脾细胞消除了对IL-2的反应，表明对IL-2有反应的细胞带有T细胞标志物。虽然未分级的胸腺细胞对IL-2没有致有丝分裂反应，但通过用Percoll进行不连续梯度离心对胸腺细胞进行分级，得到了一小部分对IL-2刺激有强烈反应的细胞群体。这些细胞分布在低密度组分中。分布在高密度组分中的细胞对IL-2无反应。
