Takahashi S, Konishi Y, Nakatani K, Inui S, Kojima K, Shiratori T
J Natl Cancer Inst. 1978 Apr;60(4):925-9. doi: 10.1093/jnci/60.4.925.
Cells (1 X 10(7)/0.5 ml) from a Borrmann type III poorly differentiated adenocarcinoma of the human stomach were injected ip into nude mice. The injection resulted in ascites carcinoma with invasion (carcinomatous peritonitis) and liver metastasis. The inoculum was obtained from subcutaneous tumors at passage 9 in nude mice that had received serial transplants from the patient with Borrmann type III poorly differentiated adenocarcinoma of the stomach. Serial transfers of 1.5 X 10(6) dispersed cancer cells/0.5 ml into the peritoneal cavity of nude mice converted this adenocarcinoma to an ascites form. Hemorrhagic ascites accumulated within 3 weeks at the first passage and 4-6 weeks in serial passages. Carcinomas peritonitis occurred consistently and was observed in the diaphragm, mesenteries, omentum, and pancreas; metastases were seen in the liver and spleen. Subsequently, iv injection of ascites at passage 3 (6 X 10(5) cells/0.2 ml) into nude mice produced metastatic lesions in the lung and the heart. The histology of the invasive and metastatic lesions in the nude mice was similar to that of the original tumor in the patient with stomach carcinoma.
将来自一名人类胃Borrmann III型低分化腺癌的细胞（1×10⁷个/0.5 ml）腹腔注射到裸鼠体内。注射导致出现伴有侵袭（癌性腹膜炎）和肝转移的腹水癌。接种物取自接受来自该胃Borrmann III型低分化腺癌患者的系列移植的裸鼠第9代皮下肿瘤。将1.5×10⁶个分散的癌细胞/0.5 ml连续转移到裸鼠腹腔内，使这种腺癌转变为腹水形式。首次传代时在3周内出现血性腹水，连续传代时在4 - 6周出现。癌性腹膜炎持续发生，见于膈肌、肠系膜、大网膜和胰腺；在肝脏和脾脏可见转移灶。随后，将第3代腹水（6×10⁵个细胞/00.2 ml）静脉注射到裸鼠体内，在肺和心脏产生转移灶。裸鼠侵袭性和转移性病变的组织学与胃癌患者的原发肿瘤相似。
