Matera L, Foa R, Moore M
J Clin Lab Immunol. 1984 Oct;15(2):85-90.
Generation of cytotoxic activity attributable to natural killer (NK)-like effector cells, against the NK-sensitive erythroleukaemia cell line K562 was estimated in peripheral blood lymphocytes (PBL) from normal donors, following stimulation with fresh leukaemic cells (FLC) of T-origin. One of 9 FLC induced anti-K562 cytotoxicity which was considerably higher than that of unstimulated control PBL. Cells derived from this patient possessed acid phosphatase (AP) and TdT activity. The reacted with the monoclonal antibodies RFA1 (directed against early and mature T cells) and A10 (specific for lymphohaemopoietic precursors). However, the cells did not form E rosettes, nor did they react with the monoclonal antibodies OKT3, OKT4, OKT8 and OKT11 to human T cell surface antigens. OKT6, which recognizes 70% of thymic lymphocytes was also negative. The high stimulatory activity of these cells may be related to the expression of surface determinants characteristic of early stages of the T-cell pathway, detected only by the monoclonal antibody RFA1.
在用源自T细胞的新鲜白血病细胞（FLC）刺激后，对正常供体的外周血淋巴细胞（PBL）中可归因于自然杀伤（NK）样效应细胞的细胞毒性活性进行了评估，该活性针对NK敏感的红白血病细胞系K562。9个FLC中有1个诱导了抗K562细胞毒性，其显著高于未刺激的对照PBL。来自该患者的细胞具有酸性磷酸酶（AP）和末端脱氧核苷酸转移酶（TdT）活性。这些细胞与单克隆抗体RFA1（针对早期和成熟T细胞）和A10（对淋巴造血前体具有特异性）发生反应。然而，这些细胞不形成E花环，也不与针对人T细胞表面抗原的单克隆抗体OKT3、OKT4、OKT8和OKT11发生反应。识别70%胸腺淋巴细胞的OKT6也呈阴性。这些细胞的高刺激活性可能与仅由单克隆抗体RFA1检测到的T细胞途径早期阶段特征性表面决定簇的表达有关。
