Miyazaki S
Microbiol Immunol. 1984;28(10):1083-92. doi: 10.1111/j.1348-0421.1984.tb00765.x.
Injection of viable cells of Flavobacterium meningosepticum at doses of 10(6) colony-forming units/eye caused ophthalmia in rabbits' corneas. Elastase, free of protease activity, was partially purified from broth cultures of F. meningosepticum ATCC12535 and TMS516. The optimum pH of the elastase activity was about 8.0. The activity was suppressed by Ni, Zn, Co, EDTA, and o-phenanthroline, and accelerated by Cu and Fe. The minimum dose of the elastase causing ophthalmia in the eyes of rabbits was 5 micrograms/eye (0.065 elastase units). The minimum lethal dose of the elastase for suckling mice by intracerebral injection was 0.4 micrograms (0.0052 elastase units)/suckling mouse, and its minimum lethal dose for adult mice by intraperitoneal injection was 740 micrograms/mouse (9.52 elastase units).
以每只眼睛10(6)个菌落形成单位的剂量注射脑膜炎败血金黄杆菌的活细胞会导致兔角膜出现眼炎。从脑膜炎败血金黄杆菌ATCC12535和TMS516的肉汤培养物中部分纯化出了无蛋白酶活性的弹性蛋白酶。弹性蛋白酶活性的最适pH约为8.0。该活性受到镍、锌、钴、乙二胺四乙酸（EDTA）和邻菲罗啉的抑制，而受到铜和铁的促进。在兔眼中引起眼炎的弹性蛋白酶的最小剂量为每只眼睛5微克（0.065个弹性蛋白酶单位）。通过脑内注射，弹性蛋白酶对乳鼠的最小致死剂量为每只乳鼠0.4微克（0.0052个弹性蛋白酶单位），通过腹腔注射，其对成年小鼠的最小致死剂量为每只小鼠740微克（9.52个弹性蛋白酶单位）。
