纯11C-葡萄糖的制备及其生物学意义

Procurement and biological significance of pure 11C-glucose.

作者信息

Saji H, Tanaka A, Magata Y, Enoki K, Tokui T, Nishihara Y, Senda M, Yonekura Y, Yokoyama A, Torizuka K

出版信息

Radioisotopes. 1984 Oct;33(10):680-5. doi: 10.3769/radioisotopes.33.10_680.

DOI:10.3769/radioisotopes.33.10_680

Abstract

Biological significance of 11C-glucose purity has been a controversial matter. In order to understand the contribution of impurities contained in the photosynthetic 11C-glucose, its analysis and biodistribution in mice was studied. Studies indicated the interference of impurities, particularly 11C-fructose in the injectate, with the quantitative evaluation of glucose metabolic rate in brain. Furthermore, results obtained offered a good basis for search of simpler and easier separation methodology. Namely, good selection and combination of commercially available high performance liquid chromatography (HPLC) column provided 11C-glucose with high radiochemical purity. The radiochemical yield achieved was 6-10% at 75 min after the end of bombardment including the HPLC purification. The imaging study by positron computed tomography in a rabbit showed the suitability of 11C-glucose as a radiopharmaceutical for the diagnosis of the brain function.

摘要

11C-葡萄糖纯度的生物学意义一直是个有争议的问题。为了了解光合11C-葡萄糖中所含杂质的影响，对其在小鼠体内的分析和生物分布进行了研究。研究表明杂质，特别是注射液中的11C-果糖，会干扰脑内葡萄糖代谢率的定量评估。此外，所获得的结果为寻找更简单易行的分离方法提供了良好的基础。也就是说，对市售高效液相色谱（HPLC）柱进行良好的选择和组合，可使11C-葡萄糖具有高放射化学纯度。包括HPLC纯化在内，轰击结束后75分钟时的放射化学产率为6-10%。在兔子身上进行的正电子计算机断层成像研究表明，11C-葡萄糖作为一种用于脑功能诊断的放射性药物是合适的。