Bonnevie-Nielsen V, Skovgaard L T, Lernmark A
Endocrinology. 1983 Mar;112(3):1049-56. doi: 10.1210/endo-112-3-1049.
The beta-cell function, total islet volume, and number were studied in 1- to 18-month-old mice, together with the extractable pancreatic insulin and glucagon. The beta-cell function, determined as the total amount of insulin released in response to glucose from the in vitro perfused pancreas showed an age-related increase, without any differences in the kinetics of insulin secretion between young and old mice. The total islet number and area in each individual pancreas was determined planimetrically after selective staining of the islets by perfusing the pancreas with dithizone. The islet area increased from 5.4 +/- 1.7 mm2 at 1 month to 16.3 +/- 2.1 mm2 at 18 months, whereas the number of islets remained virtually unchanged (1072 +/- 51). Pancreatic insulin increased with age by nearly 500%, in contrast to a 35% reduction in pancreatic glucagon. There was a strong relationship between body weight and total pancreatic DNA (P = 4.7 X 10(-8)), islet area (P = 3.2 X 10(-7)), insulin secretory capacity (P = 7 X 10(-4)), and total pancreatic insulin (P = 1.9 X 10(-5)), but no relationship between body weight and islet number. The insulin secretory capacity increased proportionally to the increase in islet area (P = 9.9 X 10(-3)). The islet area and total pancreatic insulin were closely related (P = 2.8 X 10(-12)), as were pancreatic insulin and the insulin secretory capacity (P = 3.3 X 10(-11)). There was a negative relationship between pancreatic glucagon and islet area (P = 0.005) and between pancreatic glucagon and insulin (P = 0.01). The close relationship between pancreatic insulin and islet area, shown to be an expression of islet volume, makes it possible to estimate the volume of the endocrine pancreas after standard RIA of pancreatic insulin. The combined morphometric and physiological analysis is unique in studying islet cell function relative to the volume of the endocrine pancreas.
在1至18月龄的小鼠中研究了β细胞功能、胰岛总体积和数量，同时还研究了可提取的胰腺胰岛素和胰高血糖素。β细胞功能通过体外灌注胰腺对葡萄糖反应释放的胰岛素总量来确定，结果显示其随年龄增长而增加，幼年和老年小鼠之间胰岛素分泌动力学无差异。通过用双硫腙灌注胰腺对胰岛进行选择性染色后，用平面测量法确定每个胰腺中胰岛的总数和面积。胰岛面积从1个月时的5.4±1.7mm²增加到18个月时的16.3±2.1mm²，而胰岛数量几乎保持不变（1072±51）。胰腺胰岛素随年龄增长增加近500%，相比之下，胰腺胰高血糖素减少35%。体重与胰腺总DNA（P = 4.7×10⁻⁸）、胰岛面积（P = 3.2×10⁻⁷）、胰岛素分泌能力（P = 7×10⁻⁴）和胰腺总胰岛素（P = 1.9×10⁻⁵）之间存在强相关性，但体重与胰岛数量之间无相关性。胰岛素分泌能力与胰岛面积的增加成比例增加（P = 9.9×10⁻³）。胰岛面积与胰腺总胰岛素密切相关（P = 2.8×10⁻¹²），胰腺胰岛素与胰岛素分泌能力也密切相关（P = 3.3×10⁻¹¹）。胰腺胰高血糖素与胰岛面积之间呈负相关（P = 0.005），胰腺胰高血糖素与胰岛素之间也呈负相关（P = 0.01）。胰腺胰岛素与胰岛面积之间的密切关系表明这是胰岛体积的一种表现，这使得在对胰腺胰岛素进行标准放射免疫分析后能够估计内分泌胰腺的体积。形态计量学和生理学相结合的分析在研究胰岛细胞功能与内分泌胰腺体积的关系方面具有独特性。
