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含有嗜热脂肪芽孢杆菌延伸因子的Qβ复制酶。

Q beta replicase containing a Bacillus stearothermophilus elongation factor.

作者信息

Stringfellow L, Blumenthal T

出版信息

J Bacteriol. 1983 Feb;153(2):1083-7. doi: 10.1128/jb.153.2.1083-1087.1983.

DOI:10.1128/jb.153.2.1083-1087.1983
PMID:6337116
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC221739/
Abstract

We purified Q beta replicase containing EF-Ts from Bacillus stearothermophilus in place of the homologous polypeptide from Escherichia coli. The hybrid enzyme was fully active in the transcription of a variety of templates. It was found to be qualitatively similar to native Q beta replicase with respect to a variety of parameters which measure the efficiency of initiation of RNA synthesis. The results demonstrated that Q beta replicase can tolerate substantial alterations in the EF-Tu X Ts component of the enzyme. These alterations resulted in only minor perturbations of catalytic properties.

摘要

我们从嗜热脂肪芽孢杆菌中纯化了含有EF-Ts的Qβ复制酶，以替代来自大肠杆菌的同源多肽。这种杂交酶在多种模板的转录中具有完全活性。在测量RNA合成起始效率的各种参数方面，发现它与天然Qβ复制酶在性质上相似。结果表明，Qβ复制酶能够耐受该酶的EF-Tu X Ts组分中的大量改变。这些改变仅导致催化特性的轻微扰动。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ac4/221739/5cb4b0be1182/jbacter00249-0515-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ac4/221739/5cb4b0be1182/jbacter00249-0515-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ac4/221739/5cb4b0be1182/jbacter00249-0515-a.jpg

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Q beta replicase containing a Bacillus stearothermophilus elongation factor.含有嗜热脂肪芽孢杆菌延伸因子的Qβ复制酶。
J Bacteriol. 1983 Feb;153(2):1083-7. doi: 10.1128/jb.153.2.1083-1087.1983.
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J Bacteriol. 1983 Mar;153(3):1266-71. doi: 10.1128/jb.153.3.1266-1271.1983.

本文引用的文献

1
Transfer RNA cross-linked to the elongation factor Tu subunit of Q beta replicase does not inhibit Q beta RNA replication.与Qβ复制酶的延伸因子Tu亚基交联的转运RNA不会抑制QβRNA复制。
J Biol Chem. 1981 Jun 10;256(11):5840-4.
2
Q beta replicase containing wild type and mutant tufA and tufB gene.
J Biol Chem. 1980 Jun 10;255(11):5300-5.
3
Q beta replicase template specificity: different templates require different GTP concentrations for initiation.Qβ复制酶模板特异性：不同模板起始需要不同的鸟苷三磷酸（GTP）浓度。
Proc Natl Acad Sci U S A. 1980 May;77(5):2601-5. doi: 10.1073/pnas.77.5.2601.
4
Roles of the host polypeptides in Q beta RNA replication. Host factor and ribosomal protein S1 allow initiation at reduced GTP concentration.
J Biol Chem. 1980 Dec 25;255(24):11713-6.
5
Protein synthesis elongation factors Tu and Tu.Ts from Caulobacter crescentus: sensitivity to kirromycin and activity in Q beta replicase.新月柄杆菌的蛋白质合成延伸因子Tu和Tu.Ts：对奇霉素的敏感性及在Qβ复制酶中的活性
J Bacteriol. 1980 Jul;143(1):389-95. doi: 10.1128/jb.143.1.389-395.1980.
6
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
7
Reconstitution of Q replicase lacking subunit with protein-synthesis-interference factor i.用蛋白质合成干扰因子i重建缺乏亚基的Q复制酶。
Eur J Biochem. 1972 Nov 21;31(1):44-51. doi: 10.1111/j.1432-1033.1972.tb02498.x.
8
Bacteriophage Q replicase contains the protein biosynthesis elongation factors EF Tu and EF Ts.噬菌体Q复制酶包含蛋白质生物合成延伸因子EF Tu和EF Ts。
Proc Natl Acad Sci U S A. 1972 May;69(5):1313-7. doi: 10.1073/pnas.69.5.1313.
9
Function and structure in ribonucleic acid phage Q beta ribonucleic acid replicase. The roles of the different subunits in transcription of synthetic templates.核糖核酸噬菌体Qβ核糖核酸复制酶的功能与结构。不同亚基在合成模板转录中的作用。
J Biol Chem. 1974 Sep 25;249(18):5801-8.
10
Function and structure in ribonucleic acid phage Qbeta ribonucleic acid replicase. Effect of inhibitors of EF-Tu on ribonucleic acid synthesis and renaturation of active enzyme.核糖核酸噬菌体Qβ核糖核酸复制酶的功能与结构。EF-Tu抑制剂对核糖核酸合成及活性酶复性的影响。
J Biol Chem. 1976 May 10;251(9):2749-53.