Ricketts A P, Hagensee M, Bullock D W
J Reprod Fertil. 1983 Jan;67(1):151-60. doi: 10.1530/jrf.0.0670151.
Epithelial cells and stromal cells of the rabbit endometrium were separated by successive enzymic digestion of the uterine mucosa. Isolated cell types were obtained in high yield, with good viability, and were maintained in monolayer cultures for up to 2 weeks. Epithelial cells in monolayers appeared as polygonal cells, displayed contact inhibition, and showed the presence of microvilli on the cell surface, with many desmosomes. Stromal cells grew rapidly to confluence, displayed overgrowth, and had a fibroblastic appearance with an absence of junctional complexes between cells. Indirect immunofluorescence showed uteroglobin on the surface of epithelial but not of stromal cells, and only epithelial cells secreted uteroglobin into the medium. These results confirm the identity of the cells and provide biochemical evidence for the epithelial cellular origin of uteroglobin. The method allows the culture of separate endometrial cell types, which retain their morphology and differentiated function in vitro.
通过对兔子宫内膜进行连续酶消化，分离出上皮细胞和基质细胞。分离得到的细胞类型产量高、活力好，并在单层培养中维持长达2周。单层培养的上皮细胞呈多边形，表现出接触抑制，细胞表面有微绒毛，且有许多桥粒。基质细胞迅速生长至汇合，出现过度生长，呈成纤维细胞样外观，细胞间无连接复合体。间接免疫荧光显示，子宫珠蛋白存在于上皮细胞表面而非基质细胞表面，且只有上皮细胞将子宫珠蛋白分泌到培养基中。这些结果证实了细胞的特性，并为子宫珠蛋白的上皮细胞起源提供了生化证据。该方法能够培养分离的子宫内膜细胞类型，这些细胞在体外保持其形态和分化功能。
