Chaminadas G, Propper A Y, Royez M, Prost O, Remy-Martin J P, Adessi G L
J Reprod Fertil. 1986 Jul;77(2):547-58. doi: 10.1530/jrf.0.0770547.
Epithelial and stromal cells of guinea-pig endometrium were separated by enzymic digestion, isolated by successive centrifugation, and maintained in culture as pure cell types for 5 days on growth medium. On Day 5, ultrastructural studies were performed on the two cell types, demonstrating that epithelial cells can grow as a monolayer composed of cohesive groups of polygonal cells (1.3 X 10(5) cells/cm2), while stromal cells were mostly fibroblastic. The effect of hormones was studied on the epithelial cells in culture. The monolayer was cultured into harvest medium for 3 days to ensure the complete removal of endogenous steroids, then these cells were incubated with 2 X 10(-9) M-oestradiol-17 beta for 3 days. There was a rise in the progesterone receptor level, varying from 1.3 to 10.8 times. The three enzymes known to interfere with oestradiol-17 beta metabolism were present in the epithelial cells grown in our culture conditions. By incubation with oestrone sulphate for 3 days it was demonstrated that, in cultured epithelial cells, oestrone sulphate is converted into oestradiol-17 beta sulphate, and oestrogen sulphates are hydrolysed to active oestrogens.
通过酶消化分离豚鼠子宫内膜的上皮细胞和基质细胞，经连续离心进行分离，并在生长培养基上作为纯细胞类型培养5天。在第5天，对这两种细胞类型进行了超微结构研究，结果表明上皮细胞可以生长为单层，由多边形细胞的紧密群体组成（1.3×10⁵个细胞/平方厘米），而基质细胞大多为成纤维细胞。研究了激素对培养的上皮细胞的影响。将单层细胞培养在收获培养基中3天，以确保完全去除内源性类固醇，然后将这些细胞与2×10⁻⁹M的17β-雌二醇孵育3天。孕酮受体水平有所升高，变化范围为1.3至10.8倍。在我们的培养条件下生长的上皮细胞中存在三种已知会干扰17β-雌二醇代谢的酶。通过与硫酸雌酮孵育3天表明，在培养的上皮细胞中，硫酸雌酮转化为硫酸17β-雌二醇，并且雌激素硫酸盐被水解为活性雌激素。
